Modifiye maya ekstraktı ve hscas içeren mikotoksin bağlayıcının rumende aflatoksinleri bağlama etkinliği

Bu araştırma, modifiye S. cerevisiae ve hidrate sodyum kalsiyum alüminosilikat (HSKAS) içeren mikotoksin bağlayıcının (MB) çeşitli aflatoksinlere karşı in vitro bir çalışmada ruminal bağlanma etkinliğini belirlemeyi amaçlamıştır. Sıvı formdaki sertifikalı aflatoksin karışımı (B1, G1, B2, G2) rumen sıvısıyla in vitro ortamda karıştırılarak final konsantrasyonları 6 ng/ml aflatoksin B1 (AFB1), 6 ng/ml aflatoksin G1 (AFG1), 1.5 ng/ml aflatoksin B2 (AFB2), ve 1.5 ng/ml aflatoksin G2 (AFG2) olacak şekilde hazırlanmıştır. Muamele grupları: 1) aflatoksin karışımı + distile su (Kontrol); 2) aflatoksin karışımı + rumen sıvısı (AR); ve 3) aflatoksin karışımı + MB (6 mg) + rumen sıvısı (AMBR) olarak planlanmıştır. Rumen sıvısındaki aflatoksin konsantrasyonları 39°C’deki inkübasyon zamanlarından (0, 3, 6, 12, 24 saat) sonra HPLC ile tayin edilmiştir. Her ne kadar AFB1 konsantrasyonu 0. saatte 6 ng/ml iken, bu konsantrasyon 3. ve 12. saatlerde kontrol grubunda sırasıyla 2.50 ve 1.68 ng/ml’ye, AR grubunda 0.86 ve 0.50 ng/ml’ye, AMBR grubunda ise 0.34 ve 0.20 ng/ml’ye düşmüştür (P

Efficacy of modified yeast extract and hscas containing mycotoxin adsorbent on ruminal binding characteristics of various aflatoxins

The objective of this experiment was to determine the ruminal binding characteristics of modified S. cerevisiae extract and hydrated sodium calcium aluminosilicate (HSCAS) containing mycotoxin adsorbent (MA) against various aflatoxins in an in vitro study. A certified aflatoxin mixture (B1, G1, B2, G2) in a liquid form was mixed with ruminal in vitro medium providing the final concentrations of 6 ng/ml aflatoxin B1 (AFB1), 6 ng/ml aflatoxin G1 (AFG1), 1.5 ng/ml aflatoxin B2 (AFB2), and 1.5 ng/ml aflatoxin G2 (AFG2). Treatments were: 1) aflatoxin mixture + distilled water (Control); 2) aflatoxin mixture + rumen fluid (AR); and 3) aflatoxin mixture + MA (6 mg) + rumen fluid (AMAR). After various incubation time points (0, 3, 6, 12, 24 h) at 39°C, aflatoxin concentrations in ruminal medium were detected with HPLC. Although AFB1 concentration at 0 h was 6 ng/ml, it was reduced to 2.50 and 1.68 ng/ml in Control, 0.86 and 0.50 ng/ml in AR, and 0.34 and 0.20 ng/ml in AMAR treatments at 3 and 12 h, respectively (P

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1. Van Genderen H: Adverse effects of naturally occurring non-nutritive substances. In, de Vries J (Ed): Food Safety and Toxicity. pp.153-168, CRC Press LLC, Florida, 1997.
2. Stark AA: Mechanisms of action of aflatoxin B1 at the biochemical and molecular levels. In, Wilson JL, Droby S (Eds): Microbial Food Contamination. pp. 81-94, CRC Press LLC, Florida, 2001.
3. Hussain Z, Khan MZ, Khan A, Javed I, Saleemi MK, Mahmood S, Asi MR: Residues of aflatoxin B1 in broiler meat: Effect of age and dietary aflatoxin B1 levels. Food Chem Toxicol, 48, 3304-3307, 2010.
4. Rahimi E, Bonyadian M, Rafei M, Kazemeini HR: Occurrence of aflatoxin M1 in raw milk of five dairy species in Ahvaz, Iran. Food Chem Toxicol, 48, 129-131, 2010.
5. EFSA (European Food Safety Authority): Review of mycotoxin-detoxifying agents used as feed additives: Mode of action, efficacy and feed/food safety. Scientific Report, CFP/EFSA/FEEDAP/2009/01, 2009.
6. Basmacıoğlu H, Ergül M: Yemlerde bulunan toksinler ve kontrol yolları. Hayvansal Üretim, 44, 9-17, 2003.
7. Ramos AJ, Hernandez E: Prevention of aflatoxicosis in farm animals by means of hydrated sodium calcium aluminosilicate addition to feedstuffs: a review. Anim Feed Sci Tech, 65, 197-206, 1997.
8. Yiannikouris A, Francois J, Poughon L, Dussap CG, Bertin G, Jeminet G, Jouany JP: Adsorption of zearalenone by D-glucans in the Saccharomyces cerevisiae cell wall. J Food Prot, 67, 1195-1200, 2004.
9. Kutz RE, Sampson JD, Pompeu LB, Spain JN, Vazquez-Anon M, Rottinghaus GE: Efficacy of Solis, NovasilPlus, and MTB-100 to reduce aflatoxin M1 levels in milk of early to mid lactation dairy cows fed aflatoxin B1. J Dairy Sci, 92, 3959-3963, 2009.
10. Engel VG, Hagemeister H: Untersuchungen über den verbleib von aflatoxin B1 im verdauungstrakt von kühen. Milchwissenenschaft, 33, 21-23, 1978.
11. Kiessling KH, Pettersson H, Sandholm K, Olsen M: Metabolism of aflatoxin, ochratoxin, zearalenone and three tricothecenes by intact rumen fluid, rumen protozoa and rumen bacteria. Appl Environ Microbiol, 47, 1070-1073, 1984.
12. Auerbach H, Maas RFM, Op Den Camp HJM, Pol A, Fink-Gremmels J: Biodegradation of aflatoxin B1 by bovine rumen microorganisms in vitro and its effects on rumen fermentation. Rev Med Vet, 149, 573, 1998.
13. Menke KH, Steingass H: Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Develop, 28, 7-55, 1988.
14. AOAC: Official method of Analysis. No. 999.07: Aflatoxin B1 and total aflatoxins in peanut butter, pistachio paste, fig paste and paprika powder. Immunoaffinity column-liquid chromatography with post-column derivatization. First action 1999. J AOAC Int, 83, 320, 2000.
15. Orskov ER, McDonald I: The estimation of protein degradability in the rumen from incubation measurement weighed according to rate of passage. J Agri Sci, 92, 499-503, 1979.
16. Doyle MP, Applebaum RS, Brackett RE, Marth EM: Physical, chemical and biological degradation of mycotoxins in foods and agricultural commodities. J Food Prot, 45, 964-971, 1982.
17. Upadhaya SD, Park MA, Ha JK: Mycotoxins and their biotransformation in the rumen: a review. Asian-Aust J Anim Sci, 23, 1250-1260, 2010.
18. Westlake K, Mackie RI, Dutton MF: Effects of several mycotoxins on specific growth rate of Butyrivibrio fibrisolvens and toxin degradation in vitro. Appl Environ Microbiol, 53, 613-614, 1987.
19. Nakazato M, Morozumi S, Saito K, Fujinuma K, Nishima T, Kasai N: Interconversion of aflatoxin B1 and aflatoxicol by several fungi. Appl Environ Microbiol, 56, 1465-1470, 1990.
20. Upadhaya SD, Sung HG, Lee CH, Lee SY, Kim SW, Cho KJ, Ha JK: Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats. J Vet Sci, 10, 29-34, 2009.
21. Phillips TD, Kubena LF, Harvey RB, Taylor DR, Heidelbaugh ND: Mycotoxin hazards in agriculture- new approach to control. J Am Vet Med Assoc, 190, 1617, 1987.
22. Spotti M, Fracchiolla ML, Arioli F, Caloni F, Pompa G: Aflatoxin B1 binding to sorbents in bovine ruminal fluid. Vet Res Commun, 29, 507-515, 2005.
23. Jouany JP, Yiannikouris A, Bertin G: How yeast cell wall components can alleviate mycotoxicosis in animal production and improve the safety of edible animal products. J Anim Feed Sci, 14 (Suppl. 1): 171-190, 2005.
24. Moschini M, Gallo A, Piva G, Masoero F: The effects of rumen fluid on the in vitro aflatoxin binding capacity of different sequestering agents and in vivo release of the sequestered toxin. Anim Feed Sci Tehnol, 147, 292-309, 2008.
25. Devegowda G, Aravind BIR, Morton MG: Saccharomyces cerevisiae and mannanoligosaccharides to counteract aflatoxicosis in broilers. Proc Austr Poult Sci Symp, 8, 103-106, 1996.
26. Diaz ED, Hagler WM, Hopkins BA, Whitlow LW: Aflatoxin binders I: in vitro binding assay for aflatoxin B1 by several potential sequestering agents. Mycopathologia, 156, 223-226, 2002.
27. Fink-Gremmels J: The role of mycotoxins in the health and performance of dairy cows. Vet J, 176, 84-92, 2008.