Efficacy of modified yeast extract and hscas containing mycotoxin adsorbent on ruminal binding characteristics of various aflatoxins

The objective of this experiment was to determine the ruminal binding characteristics of modified S. cerevisiae extract and hydrated sodium calcium aluminosilicate (HSCAS) containing mycotoxin adsorbent (MA) against various aflatoxins in an in vitro study. A certified aflatoxin mixture (B1, G1, B2, G2) in a liquid form was mixed with ruminal in vitro medium providing the final concentrations of 6 ng/ml aflatoxin B1 (AFB1), 6 ng/ml aflatoxin G1 (AFG1), 1.5 ng/ml aflatoxin B2 (AFB2), and 1.5 ng/ml aflatoxin G2 (AFG2). Treatments were: 1) aflatoxin mixture + distilled water (Control); 2) aflatoxin mixture + rumen fluid (AR); and 3) aflatoxin mixture + MA (6 mg) + rumen fluid (AMAR). After various incubation time points (0, 3, 6, 12, 24 h) at 39°C, aflatoxin concentrations in ruminal medium were detected with HPLC. Although AFB1 concentration at 0 h was 6 ng/ml, it was reduced to 2.50 and 1.68 ng/ml in Control, 0.86 and 0.50 ng/ml in AR, and 0.34 and 0.20 ng/ml in AMAR treatments at 3 and 12 h, respectively (P

Anahtar Kelimeler:

gaz üretimi, çiftlik hayvanları, mikotoksinler, rumen, mayalar, rumen gazları, sığır, Saccharomyces cerevisiae, aflatoksin

Modifiye maya ekstraktı ve hscas içeren mikotoksin bağlayıcının rumende aflatoksinleri bağlama etkinliği

Bu araştırma, modifiye S. cerevisiae ve hidrate sodyum kalsiyum alüminosilikat (HSKAS) içeren mikotoksin bağlayıcının (MB) çeşitli aflatoksinlere karşı in vitro bir çalışmada ruminal bağlanma etkinliğini belirlemeyi amaçlamıştır. Sıvı formdaki sertifikalı aflatoksin karışımı (B1, G1, B2, G2) rumen sıvısıyla in vitro ortamda karıştırılarak final konsantrasyonları 6 ng/ml aflatoksin B1 (AFB1), 6 ng/ml aflatoksin G1 (AFG1), 1.5 ng/ml aflatoksin B2 (AFB2), ve 1.5 ng/ml aflatoksin G2 (AFG2) olacak şekilde hazırlanmıştır. Muamele grupları: 1) aflatoksin karışımı + distile su (Kontrol); 2) aflatoksin karışımı + rumen sıvısı (AR); ve 3) aflatoksin karışımı + MB (6 mg) + rumen sıvısı (AMBR) olarak planlanmıştır. Rumen sıvısındaki aflatoksin konsantrasyonları 39°C’deki inkübasyon zamanlarından (0, 3, 6, 12, 24 saat) sonra HPLC ile tayin edilmiştir. Her ne kadar AFB1 konsantrasyonu 0. saatte 6 ng/ml iken, bu konsantrasyon 3. ve 12. saatlerde kontrol grubunda sırasıyla 2.50 ve 1.68 ng/ml’ye, AR grubunda 0.86 ve 0.50 ng/ml’ye, AMBR grubunda ise 0.34 ve 0.20 ng/ml’ye düşmüştür (P<0.001). Ayrıca AMBR grubundaki AFB1 konsantrasyonu, Kontrol ve AR gruplarıyla karşılaştırıldığında inkübasyonun 3. saatinden sonra sabit değerlere ulaşmıştır. Ancak AFB1 konsantrasyonundaki sabit değerlere ulaşma Kontrol ve AR gruplarında inkübasyonun 12. saatinden sonra gerçekleşmiştir. Benzer tipteki bağlanma şekli AMBR muamelesinde AFB2’nin ruminal inkübasyonunda da gözlenmiştir. Ayrıca AFG1 ve AFG2 konsantrasyonları, AR (0.67 ve 0.48 ng/ml) ve AMBR (0.46 ve 0.38 ng/ml) gruplarında ruminal inkübasyonun 12. saatinden sonra sabit değerlere ulaşmıştır. Kullanılan MB’nin AFG1 ve AFG2 üzerindeki bağlama kabiliyeti ruminal inkübasyonun bütün zaman dilimlerinde AMBR grubu lehine olmuştur. Muamele gruplarının rumen in vitro gaz üretimi üzerine bir etkileri gözlenmemiş ve 24. saatte ortalama 53.5 ml olmuştur. Sonuçlar MB’nin çalışılan aflatoksinleri rumen ortamında kan dolaşımına girmeden önce bağlama yeteneğine yardımcı olabileceğini ve konsantrasyonlarını düşürebileceğini göstermiştir.

Keywords:

gas production, livestock, mycotoxins, rumen, yeasts, rumen gases, cattle, Saccharomyces cerevisiae, aflatoxins,

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