Tuba İÇA, Alper ÇİFTÇİ, SERAP SAVAŞAN, Kadir Serdar DİKER

Metronidazole resistance of Campylobacter jejuni and Campylobacter coli from different animal species

Bu çalışmada çeşitli hayvanlardan izole edilen Campylobacter jejuni ve Campylobacter coli suşlarında metronidazol direnç sıklığının belirlenmesi ve karşılaştırılması amaçlandı. Çalışmada tavuk, sığır, koyun ve köpek kökenli 240 C. jejuni/C.coli izolatının metronidazol Minimum İnhibisyon Konsantrasyon (MIC) değerleri agar dilusyon tekniği ile incelendi. Direnç sıklığı 133 tavuk, 32 sığır, 48 koyun ve 27 köpek orijinli izolatlar için sırasıyla %91.7, %18.75, %16.7, ve %18.5 oranlarında bulundu. Yüz yetmiş C. jejuni izolatının %52.9’unda ve 70 C. coli suşunun %72.85’inde metronidazol dirençliliği saptandı. Farklı hayvan izolatları için metronidazol MIC (mcg/ml) aralıkları şu şekilde bulundu; tavuk, 2->64; sığır, 0.25-32, koyun, 0.12-16 ve köpek, 0.25-32. Farklı hayvan izolatları için metronidazol MIC50/MIC90 değerleri; tavuk 16/64 (mcg/ml), sığır 2/8 (mcg/ml), koyun 1/8 (mcg/ml) ve köpek 2/8 (mcg/ml) olarak tespit edildi. Çalışma sonucunda kampilobakterlerde metronidazol duyarlılığı bakımından çeşitlilik olduğu ve direnç sıklığının suşun kökenine göre değiştiği anlaşıldı. Dirençli suşların tavuklarda neden daha sık görüldüğü açıklanamamakla birlikte metronidazol dirençliliği bakımından bir konak fenotip varlığının olduğu ortaya konuldu.

Anahtar Kelimeler:

direnç, koyun, hayvan hastalıkları, metronidazol, Campylobacter coli, Campylobacter jejuni, köpek, sığır, tavuk, antibiyotikler

Farklı hayvan türlerinden izole edilen Campylobacter jejuni ve Campylobacter coli türlerinin metronidazol duyarlılıkları

In this study, we aimed to screen animal isolates of Campylobacter jejuni/Campylobacter coli for resistance to metronidazole and compared the resistance of selected groups of isolates. A total of 240 C. jejuni/C. coli isolates isolated from chickens, cattle, sheep and dogs were tested by an agar dilution technique to determine the Minimum Inhibitory Concentration (MIC) of metronidazole. The frequency of resistance in 133 avian, 32 bovine, 48 ovine and 27 canine isolates were 91.7%, 18.75%, 16.7% and 18.5%, respectively. Of 170 C. jejuni and 70 C. coli isolates tested, 52.9% and 72.85% were resistant to metronidazole, respectively. MIC (mcg/ml) ranges of metronidazole for animal isolates were as follows: chicken, 2->64; cattle, 0.25-32; sheep, 0.12-16 and dog, 0.25-32. MIC50/MIC90 values of metronidazole for animal isolates were as follows: chicken 16/64 (mcg/ml), cattle 2/8 (mcg/ml), sheep 1/8 (mcg/ml) and dog 2/8 (mcg/ml). We observed a wide range of sensitivity to metronidazole among campylobacters and frequency of resistance differed in relation to the origin of the isolate. Although it was not clear why the resistant isolates were common in chickens, a host-phenotype relationship for metronidazole resistance was demonstrated..

Keywords:

resistance, sheep, animal diseases, metronidazole, Campylobacter coli, Campylobacter jejuni, dogs, cattle, fowls, antibiotics,

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