Sirri KAR, Zafer KARAER, ESİN GÜVEN, AYŞE SERPİL NALBANTOĞLU, Ayşe ÇAKMAK, Kemal EKDAL, Asiye KOÇAK

Kanatlı coccidiosisine karşı oocystlerin irradiye edilmesi esasına dayalı aşı üretimi ııı- Eimeria spp. ve Eimeria maxima ile enfekte edilen civcivlerde oocystlerin atılım karakteristiği

Çalışmada, kanatlı coccidiosisine neden olan Eimeria türlerinin farklı yoğunluktaki enfeksiyonlarda gösterdiği atılım karakteristiklerini ortaya koymak amacıyla, her birinde 14 günlük 14 civciv bulunan 8 grup sırasıyla 25 bin, 50 bin, 100 bin, 200 bin, 350 bin, 500 bin, 750 bin ve 1 milyon ookist ile enfekte edilmiştir. Kontrol grup olarak ayrılan 9. grup ise enfekte edilmemiştir. Sahadan elde edilmiş olan ve farklı Eimeria türlerini barındıran inokulum ile gerçekleştirilen enfeksiyonu takiben, günlük olarak civcivlerin dışkıları modifiye McMaster yöntemi ile ookist yoğunluğu yönünden irdelenmiştir. Numunelerdeki E. maxima’ nın oransal yoğunluğu, morfolojik kriterler esas alınarak belirlenmiş ve aynı oransal değer üzerinden sayısal yoğunlukları hesaplanmıştır. Hayvanların takibine enfeksiyonun 30. gününde son verilmiştir. Elde edilen veriler, saha koşullarına benzer ortamlarda barındırılan ve farklı dozlarda karışık oocyst ile enfekte edilen civcivlerde, Eimeria spp ve E. maxima oocyst atılım seyrinin belli derecede enfeksiyon dozu ile ilişkili olduğunu, dönem boyunca atılan oocyst miktarının ise, gram dışkı üzerinden ele alındığında, doz ile doğrusal bir ilişkiye sahip olmadığını göstermiştir.

Anahtar Kelimeler:

Eimeria, Eimeria maxima, ookist, kanatlı hayvan hastalıkları, civciv, deneysel enfeksiyon, protozoa enfeksiyonları, koksidiyoz, aşı(hayvan), aşı geliştirme

Vaccine production based on oocyst irradiation at broiler coccidiosis ııı- characteristics of oocyst shedding in birds infected with Eimeria spp. and Eimeria maxima

The aim of this study was to expose shedding characteristics of different Eimeria spp cause coccidiosis. For this purpose, 8 groups of 14 days-old 14 birds were infected respectively with 25x103, 50x103, 100x103, 200x103, 350x103, 500x103, 750x103 and 1000x103 Eimeria spp oocysts and 9th group was determined as control. The infection carried out with the inoculum consist of different field based Eimeria spp Following the infection faecal samples were taken and examined by using the modified McMaster method in terms of oocyst density. Proportional density of E. maxima in stool samples determined base on morphological criterions and quantitative densities also calculated by using same proportional density. The data of our study showed that Eimeria spp and E. maxima dropping progress was releated with infection dose to a certain extent and discarded oocyst amount during the period was not have a linear relationship with the infection dose.

Keywords:

Eimeria, Eimeria maxima, oocysts, poultry diseases, chicks, experimental infection, protozoal infections, coccidiosis, vaccines, vaccine development,

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