Orhan YÜCEL, Adem GÜLER, MEHMET GAMSIZKAN, Nail ERSÖZ, AYŞE EKEN, Yusuf Sinan ŞİRİN, Müjdat BALKAN, Onur GENÇ

Proantosiyanidinin allograft renal oksidatif stresi önleyici etkisi: deneysel bir çalışma

Sunulan çalışma, antioksidan olan proantosiyanidinin (PC) allograft renal oksidatif stresi önlemede yararlı olabileceğini öngörerek planlandı. Hayvan materyali olarak toplam 28 rat kullanıldı ve her biri 7 rattan oluşan dört grup oluşturuldu. Birinci grup Kontrol Grup (KG): KG’daki deneklere üç gün süreyle herhangi bir işlem yapılmadan takip edildi. Üçüncü günün sonunda sol nefrektomi operasyonu yapıldı. Sol nefrektomi materyalinden histopatolojik ve biyokimyasal inceleme için dokuldan örnekleme yapıldı. İkinci Grup PC verilen grup (PCG): KG deneklere uygulanan protokolden farklı olarak üç gün takip süresinde beslenme rejimine PC eklendi. Üçüncü Grup iskemi grubu (İG): KG deneklere uygulanan protokolden farklı olarak nefrektomi materyali Wisconsin Üniversitesi (WÜ) solüsyonu içerisinde +4°C’de 24 saat bekletildikten sonra doku incelemesi için örnekleme yapıldı. Dördüncü grup tedavi grubu (TG): İG deneklere uygulanan protokolden farklı olarak üç gün takip süresinde beslenme rejimine PC eklendi. PCG, İG ve TG’larındaki deneklerin histopatolojik bulguları KG’ la benzerlik gösterdi (P>0.05). İG’unu KG’uyla karşılaştırdığımızda malondialdehid (MDA) seviyesi artmış, buna karşılık antioksidan enzimlerden superoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) aktivitelerinde düşüş kaydedildi (P

Anahtar Kelimeler:

glutatyon peroksidaz, transplantasyon, sıçan, ameliyat sonrası komplikasyonlar, böbrek hastalıkları, iskemi, histopatoloji, katalaz, allogreft

Protective effects of proanthocyanidin on allograft renal oxidative stress: an experimental study

Kidney transplantation is the first treatment option for end stage of the kidney disease. Proanthocyanidins (PC) have been reported to possess a wide range of biologic properties against oxidative stress. In this study, we aimed to investigate the effects of PC extract against ischemia related oxidative stress and injury. Rats were divided into four groups in our study. The first group served as a control (CG) was observed and fed with commercial diet for three days. Third day of observation after. The second group was proanthocyanidin given group (PCG). Proanthocyanid was added to commercial diet. The third group was ischemia group (IG). The Kidneys were preserved in UW solution around 4°C for 24 h after nephrectomy without given PC. The fourth group of animals was the treatment group (TG). PC added for feeding for three days before nephrectomy. Kidney preserved like third group. The result of malondialdehyde (MDA) levels were higher and superoxide dismutase (SOD), glutation peroxidase (GSH-Px), catalase (CAT) activities were lower in IG compared with CG (P<0.05). MDA levels were almost the same in TG and CG. Other parameters were higher in CG compared with IG (P<0.05). The administration of PC may be useful to improve ischemia related oxidative stress and injury.

Keywords:

glutathione peroxidase, transplantation, rats, postoperative complications, kidney diseases, ischaemia, histopathology, catalase, allografts,

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