Faruk ARAL, Ümit YAVUZER, ABUZER KAFAR ZONTURLU

The effect of air pressure with cervical artificial insemination on the fertility of awassi ewes synchronized with $PGF2\alpha$

Bu çalışmada, İvesi koyunlarda nativ sperma ile servikal sun’i tohumlama sırasında uygulanan hava basıncının fertilite üzerine etkisi araştırıldı. Araştırmada, aşım sezonu içerisinde bulunan ve normal siklusa sahip toplam 60 baş İvesi koyun (2-5 yaşlı) kullanıldı. Tüm koyunlara bir PGF2α analoğunun kas içi iki dozunun 7 gün arayla uygulamasını içeren PG-7 gün protokolü uygulandı. Hayvanlar iki gruba ayrıldı. Birinci gruba (n=30) sevikal sun’i tohumla yapıldı. İkinci gruba (n=30) bir hava pompası aracılığıyla servikal sun’i tohumla gerçekleştirildi. Sun’i tohumlamalar ikinci PGF2α enjeksiyonundan 48 saat sonra yapıldı. Gebelikler sun’i tohumlamayı takiben 30. günde transrektal ultrasonografi ile belirlendi. Gebelik oranı, hava pompası ile yapılan servikal sun’i tohumlamada (APCAI), servikal sun’i tohumlamadan (CAI) daha yüksek bulundu (%80.0 karşılık %46.7) (P

Anahtar Kelimeler:

sentetik prostaglandinler, suni tohumlama, atmosfer basıncı, döl verimi, basınç, pompalar, koyun ırkları, İvesi

$PGF2\alpha$ ile senkronize adilen ivesi koyunlarında hava basıncı ile servikal sun'i tohumlamanın fertiliteye etkisi

The aim of this study was to investigate the effect of air pressure on fertility during cervical artificial insemination with fresh semen in Awassi ewes. A total of 60 reproductively normal-cycling Awassi ewes (2-5 years old) were used in the experiment during the breeding season. All ewes were treated with the PG-7 days protocol consisting of two intramuscular doses of a PGF2α analogue administered 7 days apart. Animals were divided into two groups. The first group (n=30) was cervically inseminated (CAI). The second group (n=30) was cervically inseminated by means of an air pump (APCAI). Artificial inseminations were performed 48 h after the second PGF2α injection. Pregnancy was determined using ultrasonography on d 30 after AI. The pregnancy rate was found significantly higher in the APCAI group than in the CAI group (80.0% versus 46.7%) (P<0.01). The lambing rate was found to be greater in the group cervically inseminated with an air pump than in the group cervically inseminated with the conventional method (70.0 versus 46.7%). The insemination method used had a significant effect on lambing rate (P<0.05). Prolificacy was not affected by insemination methods (APCAI, 1.09; CAI, 1.14). It was concluded that cervical insemination with an air pump in Awassi ewes might improve clinical pregnancy rates by mechanisms yet to be defined.

Keywords:

synthetic prostaglandins, artificial insemination, atmospheric pressure, fertility, pressure, pumps, sheep breeds, Awassi,

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1.Sayren BL, Lewis GS: Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes. Theriogenology, 48, 267-275, 1997.

2.Paulenz H, Söderquist L, Adnøy T, Fossen HO, Berg AK: Effect of milk- and TRIS-based extenders on the fertility of sheep inseminated vaginally once or twice with liquid semen. Theriogenology, 60, 759-766, 2003.

3.Anel L, Kaabi M, Abroug B, Alvarez M, Anel E, Boixo JC, De la Fuente LF, De Paz P: Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: A field assay. Theriogenology, 63, 1235-1247, 2005.

4.O’Meara CM, Hanrahan JP, Donovan A, Fair S, Rizos D, Wade M, Boland MP, Evans ACO, Lonergan P: Relationship between in vitro fertilization of ewe oocytes and the fertility of ewes following cervical artificial insemination with frozen-thawed ram semen. Theriogenology, 64, 1797-1808, 2005.

5.Halbert GW, Dobson H, Walton JS, Buckrell BC: The structure of the cervical canal of the ewe. Theriogenology, 33, 977-992, 1990.

6.Fair S, Hanrahan JP, O’Meara CM, Duffy P, Rizos D, Wade M, Donovan A, Boland MP, Lonergan P, Evans ACO: Differences between Belclare and Suffolk ewes in fertilization rate, embryo quality and accessory sperm number after cervical or laparoscopic artificial insemination. Theriogenology, 63, 1995-2005, 2005.

7.King ME, McKelvey WAC, Dingwall WS, Matthews KP, Gebbie FE, Mylne MJA, Stewart E, Robinson JJ: Lambing rates and litter sizes following intrauterine or cervical insemination of frozen/thawed semen with or without oxytocin administration. Theriogenology, 62, 1236-1244, 2004.

8.Khalifa RM, Sayre BL, Lewis GS: Exogenous oxytocin dilates the cervix in ewes. J Anim Sci, 70, 38-42, 1992

9.Wulster-Radcliffe MC, Wang S, Lewis GS: Transcervical artificial insemination in sheep: Effects of a new transcervical artificial insemination instrument and traversing the cervix on pregnancy and lambing rates. Theriogenology, 62, 990-1002, 2004.

10.Windsor DP: Variation between ejaculates in the fertility of frozen ram semen used for cervical insemination of merino ewes. Anim Repod Sci, 47, 21-29, 1997.

11.Evans G, Maxwell WMC: Salamon’s Artificial Insemination of Sheep and Goats. Butterwoths, Sydney, 1987.

12.Salamon S, Maxwell WMC: Frozen storage of ram semen II Causes of low fertility after cervical insemination and methods of improvement. Anim Reprod Sci, 38, 1-36, 1995.

13. Gündoğan M: Short term preservation of ram semen with different extenders. Kafkas Univ Vet Fak Derg, 15, 429-435, 2009.

14. Donovan A, Hanrahan JP, Kummen E, Duffy P, Boland MP: Fertility in the ewe following cervical insemination with fresh or frozen-thawed semen at a natural or synchronised oestrus. Anim Reprod Sci, 84, 359-368, 2004.

15. Mizrachi D, Shemesh M: Follicle-stimulating hormone receptor and its messenger ribonucleic acid are present in the bovine cervix and can regulate cervical prostanoid synthesis. Biol Reprod, 61, 776-784, 1999.

16. Fuchs AR, Goeschen J, Rasmussen AB, Rehnstro¨m JV: Cervical ripening by endocervical and extra-amniotic PGE2. Prostaglandins. 28, 217-227, 1984.

17. Shemesh M, Dombrovski L, Gurevich M, Friedman S, Shore LS, Fuchs AR, Fields JF: Regulation of bovine cervical secretion of prostaglandins and synthesis of cyclooxygenase by oxytocin. Reprod Fertil Dev, 9, 525-530, 1997.

18. Leethongdee S, Khalid M, Bhatti A, Ponglowhapan S, Kershaw CM, Scaramuzzi RJ: The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period. Theriogenology, 67, 767-777, 2007.

19. Stellflug JN, Wulster-Radcliffe MC, Hensley EL, Cowardin EA, Seals RC, Lewis GS: Oxytocin-induced cervical dilation and cervical manipulation in sheep: Effects on laparoscopic artificial insemination. J Anim Sci, 79, 568-573, 2001.

20. Paulenz H, Soderquist L, Adnoy T, Nordsroga A, Berg KA: Effect of vaginal and cervical deposition of semen on the fertility of sheep inseminated with frozen-thawed semen. Vet Rec, 156, 372-375, 2005.

21. Fukui Y, Kohno H, Togari T, Hiwasa M, Okabe K: Fertility after artificial insemination using a soybean-based semen extender in sheep. J Reprod Dev, 54, 286-289, 2008.

22. Papadopoulos S, Rizos D, Duffy P, Wade M, Quinn K, Boland PM, Lonergan P: Embryo survival and recipient pregnancy rates after transfer of fresh or vitrified, in vivo or in vitro produced ovine blastocysts. Anim Reprod Sci, 74, 3544, 2002.

23. Kareta W, Korman K, Cegla M: Ovulation level and prolificacy in ewes depending on their age, birth type and percentage of prolific genotype. Reprod Biol, 6, 73-78, 2006.

24. Notter DR, Copenhaver JS: Performance of Finnish landrace crossbred ewes under accelerated lambing. I. Fertility, fecundity and ewe productivity. J Anim Sci, 51, 1033-1042, 1980.