Ali MAHDAVI, Samira KARAMATI JABEHDAR, Hamid STAJI, Farzad MIRZAEI AGHJEHGHESHLAGH, Bahman NAVIDSHAD

Fenolik Ekstrakt ve Dirençli Nişastanın Escherichia coli, Streptococcus spp., Bifidobacterium ve Lactobacillus spp. Üzerine İn-vitro Antimikrobiyal Etkisi

Bu çalışma Üzüm Posası Ekstraktının, Fıstık Kabuğu Ekstraktının ve Nar Posası Ekstraktının Dirençli Nişasta ile birlikte veya ayrı olarak in vitro şartlarda mide mikroflorası bileşenlerine bir prebiyotik olarak antimikrobiyal etkilerini araştırmak amacıyla yapılmıştır. Bu amaçla, dirençli nişasta (Fibersol 2), üzüm posası, fıstık kabuğu ve nar posası elde edilerek ekstraktları hazırlandı. Ekstraktlardaki fenolik miktarını belirlemek amacıyla Folin-Ciocalteu metodu kullanıldı. Minimum inhibe edici konsantrasyon metodu kullanılarak Escherichia coli, Streptococcus spp., Lactobacillus spp. ve Bifidobacterium spp. etkenlerine karşı ekstraktlar ± dirençli nişastanın antimikrobiyal etkisi araştırıldı. Nar posasının toplam tanin ve fenolik bileşikleri diğerlerinden daha fazlaydı. Minimum inhibe edici konsantrasyon sonuçları, 1600 ve 3200 ppm düzeyindeki fıstık kabuğu ekstraktının E. coli üremesini inhibe ettiğini gösterdi. Dirençli nişastayla birlikte Streptococcus’u büyüme inhibisyonu 400 ppm dilusyonda gerçekleşti. Streptococcus dirençli nişastayla birlikte 50, 100 ve 200 ppm fıstık kabuğu ekstraktı uygulamasında üremedi. Üzüm posası ekstraktının 800, 1600 ve 3200 ppm dozları Lactobacillus spp. üremesini önlerken Bifidobacterium spp. üremesi 3200 ppm fıstık kabuğu ekstraktı ile 50 ve 100 ppm fıstık kabuğu ekstraktı ile birlikte dirençli nişasta uygulamaları haricinde tüm uygulamalarda arttı.

In Vitro Antimicrobial Effect of Phenolic Extracts and Resistant Starch on Escherichia coli, Streptococcus spp., Bifidobacterium and Lactobacillus spp.

The present study aimed to evaluate the antimicrobial activity of Grape Pomace Extract (GPE), Pistachio Peel Extract (PsPE), andPomegranate Pomace Extract (PPE) with or without Resistant Starch (RS) as a prebiotic on gut microflora representative’s in vitroconditions. For this purpose, the Resistant Starch (Fibersol2), grape pomace, pistachio peel, and pomegranate pomace were providedand the extracts of by-products were prepared. Folin-Ciocalteu method was used to determine the total phenolic content of extracts.The antimicrobial activity of extracts ± Resistant Starch against Escherichia coli, Streptococcus spp., Lactobacillus spp. and Bifidobacteriumspp. were evaluated using Minimum Inhibitory Concentration (MIC) method. The total tannin and phenolic compounds of pomegranatepomace were more than the others. The results of MIC showed that 1600 and 3200 ppm of pistachio peel extract inhibited the E. coligrowth. The growth inhibition of Streptococcus spp. by Resistant Starch was equal to 400 ppm dilution. Streptococcus did not grow in50, 100, and 200 ppm of pistachio peel extract + Resistant Starch treatment. The dilution of 800, 1600, and 3200 ppm of grape pomaceextract could prevent the growth of Lactobacillus spp., while Bifidobacterium increased in all treatments dilution except in 3200 ppmpistachio peel extract and 50 and 100 ppm pistachio peel extract + resistant starch.

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