SENA ÇENESİZ, H. Özlem NİSBET, Gülay ÇİFTÇİ, YONCA BETİL KABAK, M. Yavuz GÜLBAHAR, Cevat NİSBET, Gul Fatma Yarim

Investigation of the effects of n-acetyl cysteine and resveratrol to acute phase response in rats with experimentally ınduced arthritis

Çalışmamızda deneysel olarak artrit oluşturulmuş ratlar üzerinde N-asetil sistein (NAS) ve reveratrolün akut faz üzerine etkileri karşılaştırıldı. Araştırmada Sprague-Dawley ırkı 30 adet yetişkin rat kullanıldı. Freund’s Complete Adjuvant (FCA) ile tüm ratların sol arka ayak pençelerine, 0.1 ml iki doz (0. ve 7. gün) intradermal injeksiyon uygulandı. Artrit oluşum sürecinin 15. gününde ratlar üç ayrı gruba ayrıldı; grup 1 ratlara %15 etil alkol i.p. grup 2 ratlara NAS i.p., grup 3 ratlara resveratrol i.p. 21 gün boyunca uygulandı. 21 günlük deneme sürecinin sonunda ratlardan kan alındıktan sonra ötanazi yapılarak eklem dokuları alındı. Biyokimyasal parametrelerden serum TNF-α, IL-1β, IL-6 düzeyleri ve ADA aktivitesinde istatistiksel olarak bir değişiklik meydana gelmezken MDA seviyesinde 3. grupta azalma (P

Anahtar Kelimeler:

tümör nekroz faktör, sıçan, hayvan modelleri, eklem yangısı, histopatoloji, enflamasyon, interleukin 1, interlökin 6, eklem hastalıkları, eklemler (hayvan), eklem sıvısı, tedavi

Deneysel olarak artrit oluşturulan ratlarda n-asetil sistein ve resveratrolün akut faz yanıta etkisinin araştırılması

The aim of the study was to investigate and compare the effects of N-acetyl cysteine (NAC) and resveratrol on acute phase response in rats with experimentally induced arthritis. In this study, 30 Sprague-Dawley mature rats were inoculated with two doses of Freund’s Complete Adjuvant (FCA) by means of intradermal injections into the left hind claws of all rats. Rats were divided into three groups at the 15th day of arthritis formation process, 15% ethyl alcohol was applied to group 1 rats via i.p., NAC was applied to group 2 rats via i.p. and resveratrol was applied to group 3 rats via i.p. for 21 days. Following the experimental period, blood was collected from the rats and then they were euthanized, and the joint tissues were collected. No significant differences were found in serum TNF-α, IL-1β, IL-6 levels and ADA activity and a decrease was determined in MDA levels in the resveratrol group. According to histopathological result, decreases in the loss of cellularity and adhesion were determined as P<0.05 and P<0.01, respectively in group received resveratrol. Compared to arthritis group, the decrease (P<0.05) in safranin-O ortochromatosis in group 2 and 3 was found. However the recovery from synovial inflammation (P<0.01) was determined both in NAC and resveratrol groups. The best recovery rate was recorded in the resveratrol group. In conclusion, resveratrol may be suggested to use in arthritis therapy alternative to NAC.

Keywords:

tumour necrosis factor, rats, animal models, arthritis, histopathology, inflammation, interleukin 1, interleukin 6, joint diseases, joints (animal), synovial fluid, therapy,

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