Bülent BÜLBÜL, Mesut KIRBAŞ, MEHMET KÖSE, ŞÜKRÜ DURSUN

Investigation of superovulation response in Brown Swiss cows after synchronization using progesterone and oestradiol valerate

Progesteron ve östradiol valerat kullanılarak yapılan senkronizasyondan sonra süperovulasyon cevabını araştırmak amacıyla 19 İsviçre Esmeri inek rastgele iki gruba ayrıldı. Kontrol grubu inekler (n=9) 10-13. günler arasında (östrüs= 0. gün) günde iki kez kas içi azalan dozlarda (4, 4, 3, 3, 2, 2, 1, 1 ml) FSH (toplam: 400 mg) enjeksiyonu ile süperovulasyona tabi tutuldu. Çalışma grubunda bulunan ineklere (n=10) ise östrüsün herhangi bir evresinde 3 mg norgestomet içeren kulak altı implantı 9 gün boyunca takıldı ve implant takıldığı gün kas içi yolla 3 mg norgestomet + 5 mg östradiol valerate enjeksiyonu yapıldı. İmplant takıldıktan sonraki 7. günden itibaren 4 gün boyunca FSH kontrol grubunda belirtildiği gibi enjekte edildi. Her iki gruptaki ineklere 5. FSH enjeksiyonu ile birlikte kas içi d-kloprostenol enjeksiyonu yapıldı. Her iki grupta da inekler östrüs başlangıcından 12 ve 24 saat sonra tohumlandılar. Ovum/embriyolar tohumlamadan 7 gün sonra toplandı. Gruplar arasında son FSH enjeksiyonu-östrüs aralığı, ortalama korpus luteum, toplam ovum/embriyo, kalite 1, 2 ve 3, transfer edilebilir ve dejenere embriyo ve fertilize olmamış ovum sayıları ve kazanım oranları açısından önemli bir fark bulunmadı (P>0.05). Elde edilen sonuçlara göre İsviçre Esmeri ineklerde süperstimülasyon öncesinde, östrüs siklusunun dönemine bakılmaksızın, 9 günlük kulak implantının uygulanma gününde progesteron+östradiol valerat enjeksiyonu ve 7. günde başlatılan süperovulasyon tedavisinin kullanılabileceği kanısına varıldı.

Anahtar Kelimeler:

sarı cisim, inek, süt sığırı, süt ineği, östradiol, FSH, hormonal kontrol, progesteron, süperovulasyon, eş zamanlama, sığır, İsviçre Esmeri(sığır ırkı)

Progesteron ve östradiol valerat kullanılarak senkronize edilen İsviçre Esmeri ineklerde süperovulasyon cevabının araştırılması

To evaluate the superovulatory response after synchronization using progesterone and oestradiol valerate, a total of 19 Brown Swiss cows were randomly allocated into two groups. Control cows (n=9) were superovulated with FSH (total: 400 mg), from d 10 to 13 (oestrus 0), using twice daily injections with decreasing doses (4, 4, 3, 3, 2, 2, 1, 1 ml). Treatment cows (n=10) were received ear implants containing 3 mg norgestomet for 9 days at random stage of the oestrus cycle, and treated intramuscularly with 3 mg norgestomet + 5 mg oestradiol valerate at the time of implant insertion. On day seven of the implant insertion, FSH was injected over a 4-d period described in the control group. D-cloprostenol was injected to cows in both groups with the 5th FSH injections. Animals in both groups were inseminated 12 and 24 h after the onset of oestrus. Seven days after the second insemination, the ova/embryos were collected. There was no significant difference between the groups for last FSH injection-oestrus intervals, the mean numbers of corpus luteum, total ova/embryos, grade 1, 2 and 3, transferable and degenerated embryos and unfertilized ova and recovery rate (P>0.05). The results confirmed that progesterone + oestradiol valerate injection at the time of insertion of an ear implant for 9 days, regardless of the stage of the oestrus cycle, can be used as a pretreatment for superstimulation started on day 7 of the implant insertion in Brown Swiss cows.

Keywords:

corpus luteum, cows, dairy cattle, dairy cows, estradiol, FSH, hormonal control, progesterone, superovulation, synchronization, cattle, Swiss Brown,

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