İnsülin-benzeri büyüme faktörü-1 ’in Japon bıldırcın embriyolarında kemik gelişimi üzerine etkileri

İnsülin-benzeri büyüme faktörü-1’in (IGF-1), Japon bıldırcın (Coturnix coturnix japonica) embriyolarının omur bölgesindeki (medulla spinalis thoracis), kemik gelişimi üzerine etkileri histolojik olarak incelendi. Rekombinant insan IGF-1‘i (rhIGF-1), yumurtanın küt ucundan, dental drill bit ile, chorio-allantoic zara zarar vermeden enjekte edildi. Kontrol ve rhIGF-1 enjekte edilen gruplar, histolojik analizler için embriyonal gelişimin 7. gününden 16. gününe kadar toplandı ve ışık mikroskobunda incelendi. Torakal kemikte, kemikleşme merkezi, rhIGF-1 uygulanan embriyolarda ilk kez 11. günde, kontrol grubunda ise 12. günde tespit edildi. Kontrol grubuyla karşılaştırıldığında, hücre sayısının rhIGF-1 enjekte edilmiş grupta önemli derecede artmış olduğu görüldü (P

Anahtar Kelimeler:

doku bilimi, insulin benzeri büyüme faktörü, büyüme, kemik, kümes hayvanları, embriyo, embriyonik gelişim, Japon bıldırcını

The effects of insulin-like growth factor-1 on bone growth during japanese quail embriyonic development

The effects of insulin-like growth factor-1 (IGF-1) on vertebrae bone (medulla spinalis thoracis) development of Japanese quails (Coturnix coturnix japonica) were histologically investigated during embriyonic development. Recombinat human IGF-1 (rhIGF-1) was injected through the blunt end of eggs via a single hole with a dental drill bit, without penetrating the chorioallantoic membrane. Control and rhIGF-1 injected groups were collected daily from days 7 to 16 of embriyonic development for histological analysis and were examined with light microscopy. Ossifi cation centers of thoracal bones were first observed on E12 for control and E11 day for rhIGF-1 injected groups, respectively. The number of cells were significantly increased in rhIGF-1 injected groups compared to that of control (P<0.05). In conclusion, the administration of rhIGF-1 accelerated the bone formation during quail embriyonic development.

Keywords:

histology, insulin-like growth factor, growth, bones, poultry, embryos, embryonic development, Japanese quails,

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