Berna Güney SARUHAN, HAKAN SAĞSÖZ, M. Aydın KETANİ, M. Erdem AKBALIK, NİHAT ÖZYURTLU

Immunohistochemical detection of estrogen and progesteron receptors in the bovine uterus and their relation to serum sex steroid hormone levels during the follicular and luteal phase

Bu çalışma, foliküler ve luteal faz süresince inek endometriumunda steroid reseptörlerinin varlığını göstermek için planlandı. Kesimhaneye getirilen 30 adet erişkin ve sağlıklı inekten hem kan hemde uterus örnekleri alındı. İmmunohistokimyasal boyamada, östrojen reseptörü için tavşan poliklonal antikoru, progesteron reseptörü için ise fare monoklonal antikoru kullanıldı. Genel olarak, uterusun bütün bölümlerindeki hücrelerinde pozitif boyanma görüldü ancak yoğunlukları farklıydı. Foliküler faz süresince, epitel ve miyometriumda hem ER hemde PR için güçlü bir boyanma vardı. Bez epitel hücrelerinde hem ER hemde PR pozitif boyanmıştı. Luteal faz süresince her iki reseptör karşılaştırıldığında, uterusun bütün bölümlerinde özellikle miyometrium ile bez ve yüzey epitelinde PR için yoğun bir boyanma görüldü. Özetle, bu çalışmanın sonuçları gösterdi ki, hem ER hemde PR, östrus siklusunun bazı dönemlerinde benzer mekanizmalar tarafından düzenlenebilir ve uterusun her bir bölümünde ER ve PR nin farklı oranlarda bulunması, onların üreme fizyolojisindeki farklı rolleri ile ilişkilidir.

Anahtar Kelimeler:

progesteron, hormon reseptörler, inek, dölyatağı, luteal hücreler, östrojen, seks hormonları, immünohistokimya

İnek uterusunda folliküler ve luteal fazda östrojen ve progesteron reseptörlerinin immunohistokimyasal olarak belirlenmesi ve serum steroidleri ile ilişkisi

In this study, we tried to show the expression patterns of the steroid receptors in the bovine endometrium during the follicular and luteal phase. Samples of both uterus and blood were obtained from 30 adult, healty bovine at the moment of slaughter at a local slaughterhouse. Immunohistochemistry was performed by using rabbit polyclonal antibodies against the estrogen receptor (ER) and mouse monoclonal antibodies against the progesterone receptor (PR). In general, most of the uterine cells were stained positive but with a different intensity. During follicular phase, both ER and PR were obviously strong in the epithelia and the myometrium. For the glandular epithelium (GE), all GE cells were stained positive for ER and PR. When we compared both receptors during lutheal phase, a stronger intensity was observed in all compartments for PR, especially in the myometrium and surface-glandular epithelium. To summarize, the results from this study showed that both ER and PR might be regulated by the same mechanisms in some compartments and at specific stages of the oestrous cycle, and that each compartment of the uterus had a different expression of ER and PR which could accord with their different roles in reproductive physiology.

Keywords:

progesterone, hormone receptors, cows, uterus, luteal cells, oestrogens, sex hormones, immunohistochemistry,

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