F. Eser ÖZGENCİL, Fikret DİRİLENOĞLU, Deniz SEYREK İNTAŞ, A. Perran GÖKÇE, Gül ÇIRAY AKBAŞ, Mehmet PİLLİ, Çağrı GÜLTEKİN, Mehmet Alper ÇETİNKAYA, Gamze MOCAN

İki Köpekte Oküler Bulaşıcı Veneral Tümör: Klinik ve Sito-histopatolojik Değerlendirme

Bulaşıcı veneral tümör (BVT), köpeklerde tipik olarak dış genital organları etkileyen bulaşıcı bir kanser türüdür. Vücudun diğer kısımlarına oto- ve heterotransplantasyon ile yayılabilir. Bu yayılım hayvanların etkileşimi sırasında, yalama ve koklama gibi, hasarlı mukozaya veya deriye ekilen neoplastik hücreler ile ya da daha az rastlanan hematojen veya lenfatik yollarla olabilir. Bu yazımızda, oküler tutulum geliştiren ve kötü prognoz gösteren iki nadir BVT olgusunun klinik, sitolojik ve histopatolojik özelliklerini sunduk. İnce iğne aspirasyon biyopsisi hızlı, minimal invaziv ve efektif olmasıyla tanıda ilk tercih edilen yöntemdi. Bu yöntem, bir olgumuzda tanısal iken, diğer olguda tanıya yardımcı oldu. İkinci olgunun sitolojik incelemesinde malign yuvarlak hücreli tümör düşünüldü ve daha ileri klinik değerlendirme ve histopatolojik inceleme ile kesin tanıya gidildi. BVT, köpeklerde oküler lezyonların ayırıcı tanısında akılda tutulması gereken yuvarlak hücreli bir tümördür.

Ocular Transmissible Venereal Tumor in Two Dogs: Clinical and Cytohistopathological Evaluation

Transmissible venereal tumor (TVT) is a transmissible cancer that typically affects the external genital organs in canines. It can spread to other parts of the body via auto- and hetero-transplantation in a way that neoplastic cells are inoculated into the degraded mucosa or skin during the interaction of the animals, such as licking and sniffing, or less commonly via hematogenous or lymphatic routes. Here, we presented the clinical, cytological, and histopathological characteristics of two rare cases of TVT that developed ocular involvement and were associated with a poor prognosis. Fine needle aspiration biopsy was the initial method of choice in the diagnosis which is rapid, minimally invasive, and effective. It was diagnostic in one case and aided in the diagnosis in the other. The latter was considered a malignant round cell tumor in the cytological examination that needed further clinical and histopathological examination for definitive diagnosis. TVT is a round cell tumor that should be considered in the differential diagnosis of ocular lesions in canines.

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1. Mello MI, Gobello C, Ferreira F: The canine transmissible venereal tumor: Etiology, pathology, diagnosis, and treatment. In, Concannon P, England G, Verstegen I (Eds): Recent Advances in Small Animal Reproduction. International Veterinary Information Service, 2005.
2. Gülbahar M, Hazıroğlu R: Bir köpekte ekstragenital metastazlı transmissible venereal tümör olgusu. Ankara Üniv Vet Fak Derg, 42, 441444, 1995.
3. Kabuusu RM, Stroup DF, Fernandez C: Risk factors and characteristics of canine transmissible venereal tumours in Grenada, West Indies. Vet Comp Oncol, 8 (1): 50-55, 2010. DOI: 10.1111/j.1476-5829.2009.00204.x
4. Park MS, Kim Y, Kang MS, Oh SY, Cho DY, Shin NS, Kim DY: Disseminated transmissible venereal tumor in a dog. J Vet Diagn Invest, 18 (1): 130-133, 2006. DOI: 10.1177/104063870601800123
5. Mozos E, Méndez A, Gómez-Villamandos JC, De Las Mulas JM, Pérez J: Immunohistochemical characterization of canine transmissible venereal tumor. Vet Pathol, 33 (3): 257-263, 1996.
6. Miller W, Albert R, Boosinger T: Ocular metastasis of a transmissible venereal tumour. Canine Pract, 15 (3): 19-21, 1990.
7. Featherstone H, Heinrich C, Donaldson D, Hartley C, Dietrich U, Ekesten B: Eye examination and diagnostics. In, Gelatt K (Ed): Essentials of Veterinary Ophthalmology. 103-145, Blackwell published, Ames, Iowa, USA, 2014.
8. Milo J, Snead E: A case of ocular canine transmissible venereal tumor. Can Vet J, 55 (1): 1245-1249, 2014.
9. Ferreira AJA, Jaggy A, Varejäo AP, Ferreira MLP, Correia JMJ, Mullas JM, Almeida O, Oliveira P, Prada J: Brain and ocular metastases from a transmissible venereal tumour in a dog. J Small Anim Pract, 41 (4): 165168, 2000. DOI: 10.1111/j.1748-5827.2000.tb03187.x
10. Das U, Das AK: Review of canine transmissible venereal sarcoma. Vet Res Commun, 24, 545-556, 2000. DOI: 10.1023/a:1006491918910
11. Amstutz H, Archibald J, Armour J, Blood D, Newberne, PM Snoeyenbos G: Diseases of the reproductive system transmissible canine venereal tumor. In, Fraser C, Mays A (Eds): The Merck Veterinary Manual. 662-663, Rahway, Merck & Co. Inc, New Jersey, USA, 1986.
12. Chikweto A, Kumthekar S, Larkin H, Deallie C, Tiwari KP, Sharma RN, Bhaiyat MI: Genital and extragenital canine transmissible venereal tumor in dogs in Grenada, West Indies. Open J Vet Med, 3 (2): 111-114, 2013. DOI: 10.4236/ojvm.2013.32018
13. Pereira JS, Silva ABF, Martins ALB, Ferreira AMR, Brooks DE: Immunohistochemical characterization of intraocular metastasis of a canine transmissible venereal tumor. Vet Ophthalmol, 3 (1): 43-47, 2000. DOI: 10.1046/j.1463-5224.2000.00097.x
14. Komnenou AT, Thomas ALN, Kyriazis AP, Poutahidis T, Papazoglou LG: Ocular manifestations of canine transmissible venereal tumour: A retrospective study of 25 cases in Greece. Vet Rec, 176 (20): 523, 2015. DOI: 10.1136/vr.102968
15. Rodrigues GN, Alessi AC, Laus JL: Intraocular transmissible venereal tumor in a dog. Ciênc Rural, 31 (1): 141-143, 2001. DOI: 10.1590/S010384782001000100023
16. Barron CN, Saunders LZ, Seibold HR, Heath MK: Intraocular tumors in animals. V. Transmissible venereal tumor of dogs. Am J Vet Res, 24, 12631270, 1963.
17. Pennick D, D’Anjou MA: Atlas of Small Animal Ultrasonography. Wiley and Blackwell, USA, 2008.
18. Veloso JF, de Andrade Oliveira TN, Andrade LP, Silva FL, Sampaio KMOR, Michel AFRM, de Lavor MSL, Carlos RSA: Three cases of exclusively extragenital canine transmissible venereal tumor (cTVT). Acta Sci Vet, 46 (Suppl. 1): 295, 2018. DOI: 10.22456/1679-9216.86846
19. Crossley R, Ramírez JA: Tumor venéreo transmisible canino de presentación atípica. Rev Med Vet Zoot, 64 (3): 78-90, 2017. DOI: 10.15446/ rfmvz.v64n3.68695
20. Thangathurai R, Balasubramaniam GA, Dharmaceelan S, Balachandran P, Srinivasan P, Sivaseelan S, Manohar BM: Cytological diagnosis and its histological correlation in canine transmissible venereal tumour. Vet Arhiv, 78 (5): 369-376, 2008.
21. Hernández-Granados AJ, Franco-Molina MA, Coronado-Cerda EE, Zapata-Benavides P, Gamboa EM, Ramos-Zayas Y, SantanaKrímskaya SE, Rodríguez-Padilla C: Immunogenic potential of three transmissible venereal tumor cell lysates to prime canine-dendritic cells for cancer immunotherapy. Res Vet Sci, 121, 23-30, 2018. DOI: 10.1016/j. rvsc.2018.10.001
22. Vural SA, Haziroglu R, Vural MR, Polat IM, Tunc AS: Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy. J Vet Sci, 19 (5): 620-626, 2018.
23. Den Otter W, Hack M, Jacobs JJL, Tan JFV, Rozendaal L, Van Moorselaar RJA: Effective treatment of transmissible venereal tumors in dogs with Vincristine and IL2. Anticancer Res, 35, 3385-3392, 2015.
24. Den Otter W, Hack M, Jacobs JJL, Tan JFV, Rozendaal L, Van Moorselaar RJA: Treatment of transmissible venereal tumors in dogs with intratumoral interleukin-2 (IL-2). A pilot study. Anticancer Res, 35, 713-718, 2015.
25. Setthawongsin C, Techangamsuwan S, Tangkawattana S, Rungsipipat A: Cell-based polymerase chain reaction for canine transmissible venereal tumor (CTVT) diagnosis. J Vet Med Sci, 78 (7): 1167-1173, 2016. DOI: 10.1292/jvms.15-0710