Ali GÜCÜKOĞLU, Tolga UYANIK, Özgür ÇADIRCI, Göknur TERZİ GÜLEL, Sibel KANAT

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Bu çalışmada, 240 organik tavuk parça eti (80 but, 80 kanat, 80 derisiz-göğüs eti) Listeria monocytogenes varlığı yönünden analiz edildi. Analiz bulguları çerçevesinde; toplam 240 örneğin 60’ında (%25) L. monocytogenes saptandı. Bulgularının örneklere göre dağılımı incelendiğinde; but örneklerinin 24’ünde (24/80-%30), kanat örneklerinin 20’sında (20/80-%25), derisiz göğüs eti örneklerinin ise 16’sında (16/80-%20) L. monocytogenes tespit edildi. Serotip dağılımında ise but örneklerinden elde edilen 40 L. monocytogenes izolatının 31’inin L. monocytogenes 1/2a, 6’sının L. monocytogenes 1/2b, 3’ünün L. monocytogenes 1/2c, 1’inin ise L. monocytogenes 4b serotipinde olduğu, kanat örneklerinden elde edilen 34 L. monocytogenes izolatının 24’ünün L. monocytogenes 1/2a, 9’unun L. monocytogenes 1/2b, 1’inin ise L. monocytogenes 4b serotipi olduğu, derisiz göğüs eti örneklerinden elde edilen 22 L. monocytogenes izolatının 14’ünün L. monocytogenes 1/2a, 6’sının L. monocytogenes 1/2b, 2’sinin ise L. monocytogenes 4b serotipi olduğu belirlendi. Antibiyotik direnç profiline bakıldığında; 26 izolat (%27) ampisiline dirençli bulunurken, meropenem, tetrasiklin, sülfametoksazol/trimetoprim, penisilin G, amoksisilin/klavulanik asit, vankomisin, oksitetrasiklin, eritromisin ve kloramfenikole karşı dirençli izolat sayısı sırası ile 23 (%23.9), 14 (%14.5), 13 (%13.5), 12 (%12.5), 9 (%9.3), 7 (%7.2), 5 (%5.2), 4 (%4.1) ve 3 (%3.1) olarak saptandı. L. monocytogenes izolatlarının 12’sinde ise çoklu antibiyotik direnç profili belirlendi. Sonuç olarak bu çalışmada tespit edilen bulguların L. monocytogenes’in organik kanatlı etlerinde epidemiyolojisini ortaya koyacak serotiplendirme çalışmalarına, gıda sektöründe faaliyet gösteren işletmelere ve listeriyosizin tanı ve tedavisinde özgün veri niteliğinde olacağı düşünülmektedir.

Serotyping and Antibiotic Resistance Profile of Listeria monocytogenes Isolated from Organic Chicken Meat

In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respectively. Multiple antibiotic resistance profiles were determined in 12 of L. monocytogenes isolates. The findings of this study are thought to be unique data for serotyping studies that will help in revealing the epidemiology of L. monocytogenes in organic poultry meat, enterprises operating in food sector and diagnosis and treatment of listeriosis.

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