Lin-sheng GUI, Zhi-you WANG, Jian-lei JIA, Cheng-tu ZHANG, Yong-zhong CHEN, Sheng-zhen HOU

IGF-1 Gene Polymorphisms Influence Bovine Growth Traits in Chinese Qinchuan Cattle

Insulin-like growth factor I (IGF-1) influences gonadotrophin-releasing hormone (GnRH) neurons during puberty, and plays an important role in muscle growth in farm animals. The objective of this study was to estimate the genotype frequencies of the polymorphisms of IGF-1 and to determine associations between these polymorphisms and growth traits in Chinese Qinchuan cattle breeds. Five single nucleotide polymorphisms (SNPs), including one in intron 2 (g.5172T>G) and four in intron 3 (g.56495C>A, g.56501C>T, g.56801C>T and g.71090T>G) were identified according to the sequencing results of 487 individual of a Qinchuan cattle population. The chi-square test showed g.5172T>G, g.56495C>A and g.56801C>T were in Hardy-Weinberg equilibrium in all the samples. Six different haplotypes were identified, of which two major haplotypes had a frequency of 48.2% (-TCTTT-) and 16.3% (-TCCTT-). The single marker association analysis showed that the three polymorphisms (g.5172T>G, g.56501C>T and g.71090T>G) were significantly associated with growth traits (at P

IGF-1 Gen Polimorfizmi Çin Qinchuan Sığırında Büyüme Özelliklerini Etkiler

İnsülin benzeri büyüme faktörü (IGF-1) çiftlik hayvanlarında gelişme döneminde gonadotropin salgılatıcı hormon nöronlarını etkiler ve kas gelişiminde önemli bir rol oynar. Bu çalışmanın amacı, Çin Qinchuan sığırında IGF-1 polimorfizminin genotip çeşitliliğini tespit ederek büyüme özellikleri üzerine etkisini belirlemektir. Qinchuan sığır popülasyonunda 487 sığırın sekans sonuçlarına göre, intron 2’de bir adet (g.5172T>G) ve intron 3’de dört adet (g.56495C>A, g.56501C>T, g.56801C>T and g.71090T>G) olmak üzere toplam beş adet tek nükleotid polimorfizmi tespit edildi. Ki kare testine göre, g.5172T>G, g.56495C>A ve g.56801C>T tüm örneklerde Hardy-Weinberg eşitliğindeydi. Altı farklı haplotip tespit edildi ve bunlardan iki ana haplotip %48.2 (-TCTTT-) ve %16.3 (-TCCTT-) sıklıkta gözlemlendi. Tek marker asosiasyon analizi, üç polimorfizmin (g.5172T>G, g.56501C>T ve g.71090T>G) anlamlı derecede olmak üzere büyüme özellikleri ile ilişkili olduğunu gösterdi (P

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