MEHMET TOLGA TAN, YAKUP YILDIRIM, MAHMUT SÖZMEN, SEVAL BİLGE DAĞALP, VOLKAN YILMAZ, ALİ HAYDAR KIRMIZIGÜL, Erhan GÖKÇE

A histopathological, immunohistochemical and molecular study of cutaneous bovine papillomatosis

Türe özgü 12 farklı genotipi tanımlanmış sığır papillomavirusları (BPV), hayvanlar ve insanlarda deri papillomlarının bazı formlarına neden olurlar. Sığırlarda BPV-1 ve BPV-2 ile enfekte dermal fibroblast ve epidermal keratonositlerin aşırı çoğalması sonucunda fibropapillomlar oluşmaktadır. Bu çalışmada sığırların deri tümörlerinde etiyolojik ajanlardan biri olan papilloma viruslarının rolü araştırılmıştır. Papillomların yaygın olarak bulunduğu sürülerde, BPV enfeksiyonunun teşhisi amacıyla; rasgele seçilen multiple deri tümörleri bulunan beş sığırdan örnekler alındı. Araştırmada papilloma enfeksiyonunun teşhisine yönelik olarak dünyada uygulanırlığı kabul edilen histopatolojik, immunohistokimyasal ve moleküler yöntemler kullanıldı. Histopatolojik ve immunohistokimyasal metotlarla deri fibropapillomatozisleri doğrulandıktan sonra, sığır deri fibropapillomatozislerine neden olan BPV-1 ve BPV-2 nin rolü, üç çiftlikten alınan beş sığır örneğinde moleküler olarak PZR (polimeraz zincir reaksiyonu) yöntemi ile araştırıldı. Sonuçta PZR metodu ile BPV-1’in DNA zincirindeki kontrol bölgesi tespit edilmesine rağmen BPV-2 negatif bulundu. Etiyolojik nedeni ortaya konan bu araştırma ile enfeksiyonun yaygınlığına bağlı ekonomik girdiler değerlendirildiğinde çalışma sonucunda, aşı çalışmalarının ve koruyucu tedbirlerin önemi ön plana çıkmaktadır.

Anahtar Kelimeler:

DNA, sığır papilloma virüsü, sığır, teşhis, histopatoloji, immünohistokimya, polimeraz zincir reaksiyonu, viral hastalıklar

Sığır deri papillomatozisinin histopatolojik, immunohistokimyasal ve moleküler araştırılması

In animals and man, some forms of cutaneous papillomas are caused by papillomaviruses. Twelve different genotypes of bovine papillomavirus (BPV) have been identified. BPV-1 through BPV-12 are all strictly species-specific. BPV-1 and BPV-2 are associated with fibropapillomas in cattle; these tumors are formed by excessive proliferation of virus-infected dermal fibroblasts and epidermal keratinocytes. In the present study well known diagnostic procedures including histopathological, immunohistochemical and molecular methods were used for the diagnosis of bovine papillomavirus infection.Samples were taken randomly from five cattle with multiple cutaneous tumor formations for the diagnosis of bovine papillomavirus infection occurring commonly in herds.In the present study five cattle were examined for the presence of multiple cutaneous tumors. Cutaneous fibropapillomatosis were confirmed histopathologically and immunohistochemically. BPV-1 and BPV-2 which are etiological agents for the bovine cutaneous fibropapillomatosis were investigated by PCR. BPV serotyping was performed from five cattle all coming from three different farms. In conclusion papillomaviral DNA was detected by the PCR. The amplified long control region (LCR) DNA sequence was identical to that of BPV-1 and negative for the BPV-2 DNA. When considered economical impact of the disease associated with the abundance of the infection significance of protective measures and vaccine studies come forward.

Keywords:

DNA, bovine papillomavirus, cattle, diagnosis, histopathology, immunohistochemistry, polymerase chain reaction, viral diseases,

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