Zhao Xiong WANG, Shi Min GAO, Qiao wei ZHENG, Min Hua SUN, Wei xin JIA, Tao REN

Epidemiology and evolutionary characteristics of avian infectious bronchitis virus in China

To examine the epidemiology and evolutionary characteristics of avian infectious bronchitis virus (IBV) in mainland China, the S1 gene of 63 IBVs isolated after 1993 was amplified by reverse transcription (RT) –polymerase chain reaction (PCR) and sequenced. Our results showed that the sequence length of 63 IBV strains ranged from 1.608 to 1.635 nucleotide (nt). Compared to the published representative strains, the homology of S1 nt sequences and their deduced amino acid (aa) residues between these isolates and reference strains ranged from 74.6% to 99.9% and 71.7% to 100%, respectively. Analysis of S proteins demonstrated that 63 isolates had eight kinds of cleavage sites (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR and RRLRR), and the amount of RRFRR and HRRRR reached 46. Furthermore, the study showed that there was no direct relation between cleavage sites and genotypes, and cleavage sites were unable to decide the pathology types. Compared to vaccine strain H120, S1 nt sequences of 63 isolates had four sites deleted or inserted frequently, and the S1 proteins had three hypervariable regions. A comprehensive study was also carried out to study the S1 nt sequences of 63 isolates, 210 published reference strains and 10 vaccine strains; the investigation showed that all these stains could be divided into two types including 11 kinds of genotypes by the phylogenetic analysis. However, 63 isolates belonged to Mass, American, LX4, LHLJ/95 I, LDT3/03, J, BJ and LDL/97 I genotypes, and the LX4 genotype was co-circulated dominantly in chicken flocks over an 18-year period. It indicated that there were several IBV genotypes with new changes circulating in China, emphasizing the importance of continued IBV surveillance.

Çin'de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri

Avian Enfeksiyöz Bronşitis Virüsünün (IBV) epidemiyolojisini ve evrimsel ezelliklerini araştırmak amacıyla Çin’de 1993 yılından sonra izole edilen 63 IBV S1 geni ters transkriptaz (RT)-polimeraz zincir reaksiyonu (PCR) ile amlifiye edilerek sekansı yapıldı. Çalışma sonuçları 63 IBV suşunun sekans boyutunun 1.608 ile 1.635 nükleotide (nt) arasında değiştiğini gösterdi. Yayınlanmış benzer suşlar ile karşılaştırıldığında bu izolatlar ile referans suşları arasında S1 nt sekanslarının homolojisi ve açığa çıkan amino asit (aa) rezidüleri sırasıyla %74.6 ile %99.9 ve %71.7 ile %100 arasında değişti. S proteinlerinin analizi 63 izolatın 8 çeşit bölünme bölgesine (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR ve RRLRR) sahip olduğunu ve RRFRR ve HRRRR miktarının 46’ya ulaştığını ortaya koydu. Ayrıca, bölünme bölgesi ile genotipler arasında doğrudan bir ilişki olmadığı ve bölünme bölgelerinin patoloji tiplerini belirlemediği tespit edildi. Aşı suşu H120 ile karşılaştırıldığında 63 izolatın S1 nt sekanslarının sıklıkla çıkarılmış veya eklenmiş 4 bölgeye sahip olduğu belirlendi. S1 proteinleri üç adet oldukça değişken bölgeye sahip olduğu gözlemlendi. 63 izolatın S1 nt sekansları, 210 yayınlanmış referans suşu ve 10 aşı suşunu araştırmak amacıyla ayrıca geniş çaplı bir çalışma yürütüldü. Tüm bu suşlar filogenetik analiz ile 11 çeşit genotipi de içeren iki tipe ayrılabilir. Ancak, 63 izolat Mass, American, LX4, LHLJ/95 I, LDT3/03, J, BJ ve LDL/97 I genotiplerine aitti ve LX4 genotipinin 18 yıldan daha uzun süredir tavuk sürülerinde baskın olarak bulunduğu belirlendi. Bu çalışma ile Çin’de yeni değişimlerle birlikte birkaç IBV genotipinin bulunduğu ve sürekli takibin önemi ortaya konuldu

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