H. Kaan MÜŞTAK, BARIŞ SAREYYÜPOĞLU, K. Serdar DIKER

High-level mupirocin resistance in a Staphylococcus pseudintermedius strain from canine origin

Mupirosin insan ve hayvanlarda özellikle metisilin dirençli Staphylococcus pseudintermediusun dekolonizasyonuna ve burun taşıyıcılığına karşı kullanılan önemli bir antibakteriyel ajandır. Bu çalışmada, piyodermalı bir köpekten S. pseudintermedius izole edildi. Bu suşun moleküler teşhisi için pta geni MboI enzimi ile kesildi. Yüksek seviye mupirosin direnci agar dilusyon metodu ve ile S-2 geninin çoğaltılmasıyla ortaya kondu. S. pseudintermedius un 16S rRNA ve ile S-2 genlerinin dizi analizi yapılarak her iki genin sekansları GenBanka kayıt ettirildi. Bu rapor, Türkiyede piyodermalı bir köpekten izole edilen ilk yüksek seviye mupirosin dirençli S. pseudintermedius olgusunu bildirmektedir.

Köpek kökenli bir Staphylococcus pseudintermedius suşunda yüksek seviye mupirosin direnci olgusu

Mupirocin is an important antibacterial agent used in both humans and animals topically, especially against nasal carriage and decolonization of methicillin-resistant Staphylococcus pseudintermedius . In this report, a S. pseudintermedius strain was isolated from a dog with pyoderma. Molecular identification of this strain was performed by digestion of pta gene with MboI enzyme. High-level mupirocin resistance was determined by agar dilution method and amplification of ile S-2 gene. Sequence analysis of 16S rRNA and ile S-2 gene of S. pseudintermedius was also performed and both sequences were accessed in GenBank. This is the first report of a high- level mupirocin resistant S. pseudintermedius strain from a dog with pyoderma in Turkey.

PDF

___

1. Loefer A, Linek M, Moodley A, Guardabassi L, Sung JM, Winkler M, Weiss R, Lloyd DH: First report of multiresistant, mecA-positive Staphylococcus intermedius in Europe: 12 cases from a veterinary derma tology referral clinic in Germany. Vet Dermatol, 18, 412-421, 2007.
2. Vincze S, Paasch A, Walther B, Ruscher C, Lübke-Becker A, Wieler LH, Barbara K: Multidrug- and methicillin resistant Staphylococcus pseudintermedius as a cause of canine pyoderma: A case report. Berl Munch Tierarztl Wochenschr, 123 (9-10): 353-358, 2010.
3. Van Hoovels L, Vankeerberghen A, Boel A, Van Vaerenbergh K, De Beenhouwer H: First case of Staphylococcus pseudintermedius infection in a human. J Clin Microbiol, 44, 4609-4612, 2006.
4. Frank LA, Loefer A: Meticillin-resistant Staphylococcus pseudintermedius: Clinical challenge and treatment options. Vet Dermatol, 23, 283-e56, 2012.
5. Thomas CM, Hothersall J, Willis CL, Simpson TJ: Resistance to and synthesis of the antibiotic mupirocin. Nat Rev Microbiol, 8 (4): 281-289, 2010.
6. Bannoehr J, Franco A, Iurescia M, Battisti A, Fitzgerald JR: Molecular diagnostic identification of Staphylococcus pseudintermedius. J Clin Microbiol, 47, 469-471, 2009.
7. Ardıç N, Özyurt M, Sareyyüpoğlu B, Haznedaroğlu T: Investigation of erythromycin and tetracycline resistance genes in methicillin-resistant staphylococci . Int J Antimicrob Agents, 26, 213-218, 2005.
8. Clinical and Laboratory Standards Institute: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Eight Edition: Approved Standard M07-A8. CLSI, Wayne, PA, USA, 2009.
9. Anthony RM, Connor AM, Power EGM, French GL: Use of the polymerase chain reaction for rapid detection of high-level mupirocin resistance in staphylococci. Eur J Clin Microbiol Infect Dis, 18, 30-34, 1999.
10. Loefer A, Baines SJ, Toleman MS, Felmingham D, Milsom SK, Edwards EA, Lloyd DH: In vitro activity of fusidic acid and mupirocin against coagulase-positive staphylococci from pets. J Antimicrob Chemother, 62, 1301-1304, 2008.
11. Hurdle JG, ONeill AJ, Mody L, Chopra I, Bradley SF: In vivo transfer of high-level mupirocin resistance from Staphylococcus epidermidis to methicillin-resistant Staphylococcus aureus associated with failure of mu pirocin prophylaxis. J Antimicrob Chemother, 56, 1166-1168, 2005.