Ebru YILMAZ

Mutagenecity of Enniatin A1 and B1 Mycotoxins in Ames Salmonella microsome test

Mikotoksinler Aspergillus, Penicillium, Fusarium, Alternaria gibi bazı mikrofunguslar tarafından üretilen sekonder meta- bolitlerdir. Enniatinler birkaç Fusarium suşu tarafından üretilir ve siklik peptid mikotoksinlerdir. Enniatinler antifungal, anti- bakteriyel ajan ve enzim inhibitörü olarak hareket etmek gibi biyolojik aktivitelere sahiptir. Bu çalışmada Enniatin A1 ve B1in mutajenik etkileri Ames testiyle araştırıldı. Ames hücresel seviyedeki mutasyonları belirleyen bir test sistemidir. Bu testte Salmonella typhimurium TA98 ve Salmonella typhimurium TA100 suşları metabolik enzim olan S9lu ortamda ve S9suz ortamda kullanıldı. Enniatin A1 ve B1in 5 farklı konsantrasyonu (12.5 µM, 25 µM, 50 µM, 100 µM, 200 µM) bu suşlara uygulandı. Bütün test suşlarında(Salmonella typhimurium TA98 ve Salmonella typhimurium TA100) Enniatin A1 ve B1 mutajenik etki göstermedi.

Enniatin A1 ve B1 mikotoksinlerinin Ames Salmonella mikrozom testi ile mutajenitesi

Mycotoxins are secondary metabolites produced by microfungi like Aspergillus, Penicillium, Fusarium, Alternaria . The enniatins are cyclic peptide mycotoxins and have produced by several strains of Fusarium sp. They have got biological activities like acting as enzyme inhibitors, antifungal and antibacterial agents. In this research the mutagenic effects of enniatin A1 and B1 were investigated with Ames assay. Ames is a test system which can detect mutations at celluler level. In this test Salmonella typhimurium TA98 and Salmonella typhimurium TA100, both in the presence and absence of S9 metabolic activation was used. Five different concentrations of Enniatin A1 and B1 (12.5 µM, 25 µM, 50 µM, 100 µM, 200 µM) were exposed to these strains. In all test strains (Salmonella typhimurium TA98 and Salmonella typhimurium TA100) Enniatin A1 and B1 did not show mutagenic effect.

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