Zhao Xiong WANG, Xian Wei LI, Min Hua SUN, Shi Min GAO, Da Wei LIU, Jing HE, Tao REN

Genotypic and pathogenic Characterization of Newcastle Disease Viruses isolated from domestic ducks in China

Çinin Guangdong Bölgesindeki ördeklerde Newcastle Hastalığı Virusu (NDV) prevalansını belirlemek amacıyla evcil ördeklerden 10 NDV izole edildi. MDT, ICPI ve F proteininin ayrılma yeri dikkate alınarak sekiz izolatın patojenik olduğu belirlendi. 10 izolatın F genlerinin sekansları yapıldı, tüm genler ve onlara ait amino asitler 32 referans türleri ile karşılaştırıldı. 5 izolat genotip VIIde toplanırken (VIId), 3 izolat genotip IX ve 2 izolat genotip olarak sınıfandırıldı. Subgenotip VIID izolatları 112R-R-Q-K-R-F117 motifine sahipken genotip IX izolatları 112R-R-Q-R-R-F117 motifi gösterdi. 8 NDV izolatı yüksek ICPI sergilerken bunlar NDVnin velojenik tipi olarak sınıfandırıldı ve bu örnekler 2006 ile 2010 yılları arasında toplandı. 112G-K-Q-G-R-L117 motifine sahip olan genotip I izolatları 2005 yılında toplandı. Bunlar düşük ICPI gösterdi ve NDVnin lentojenik tipi olarak sınıfandırıldı. Subgenotip VIInin NDVleri şimdi dominant olup Guangdong Bölgesindeki ördek çiftliklerinde son zamanlardaki çoğu ND salgınlarında belirlenmiştir. Bu bulgular Çinde ördeklerdeki NDVün genetik ve moleküler gelişimi hakkında bilgi vermekte ve hastalığın kontrol stratejileri hakkında NDV takibinin önemini ortaya koymaktadır.

Çinde evcil ördeklerden izole edilen Newcastle Hastalığı Viruslarının genotipik ve patojenik karakterizasyonu

In order to determine the prevalence of Newcaste Disease Virus (NDV) in ducks in Guangdong province of China, 10 NDVs were isolated in domestic ducks. Eight isolates were pathogenic as determined by MDT, ICPI and cleavage site of F protein. The F genes of 10 isolates were sequenced, all the genes and their deduced amino acids were compared with 32 reference strains. 5 isolates were clustered in genotype VII (VIId), 3 isolates were classified as genotype IX and 2 isolates were classified as genotype. The subgenotype VIId isolates possessed the motif 112R-R-Q-K-R-F117, and the genotype IX isolates possessed the motif 112R-R-Q-R-R-F117. The 8 NDV isolates exhibited a high ICPI, they were classified as velogenic type of NDVs and were collected between the years of 2006 and 2010. The genotype I isolates possessing the motif 112G-K-Q-G-R-L117 were collected in 2005, they were exhibited a low ICPI and were classified as lentogenic type of NDVs. The NDVs of subgenotype VII are now dominant and have been implicated in most of the recent ND outbreaks in duck farms in Guangdong province. These findings provide data on genetics and molecular evolution of NDV in ducks in China and emphasize importance of NDV surveillance for improving of strategies for the control of the disease.

PDF

___

1. Alexander DJ: Newcastle disease and other avian paramyxoviruses. Rev Sci Tech - Office International des Epizooties, 19, 443-462, 2000.
2. Werner O, Oberdorfer A, Kollner B: Characterization of avian paramyxovirus type I strains isolated in Germany during 1992 to 1996. Avian Pathol, 28, 79-88, 1999.
3. Czegledi A, Ujvari D, Somogyi E, Wehmann E, Werner O, Lomniczi B: Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications. Virus Res, 120, 36-48, 2006.
4. de Leeuw O, Peeters B: Complete nucleotide sequence of Newcastle disease virus: Evidence for the existence of a new genus within the subfamily paramyxovirinae. J Gen Virol, 80, 131-136, 1999.
5. Kolakofsky D, Roux L, Garcin D, Ruigrok RW: Paramyxovirus mRNA editing, the rule of six and error catastrophe: A hypothesis. J Gen Virol, 86, 1869-1877, 2005.
6. Steward M, Vipond IB, Millar NS, Emmerson PT: RNA editing in Newcastle disease virus. J Gen Virol, 74, 2539-2547, 1993.
7. Kim LM, King DJ, Curry PE, Suarez DL, Swayne DE: Phylogenetic diversity among low-virulence newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates. J Virol, 81, 12641-12653, 2007.
8. Liu XF, Wan HQ, Ni XX, Wu YT, Liu WB: Pathotypical and genotypical characterization of strains of Newcastle disease virus isolated from outbreaks in chicken and goose focks in some regions of China during 1985-2001. Arch Virol, 148, 1387-1403, 2003.
9. Patti JM, Eduardo LD, Claudio LA: Newcastle disease: Evolution of genotypes and the related diagnostic challenges. Infect Genet Evol, 10, 26-35, 2010.
10. Marin MC, Villegas P, Bennett JD, Seal BS: Virus characterization and sequence of the fusion protein gene cleavage site of recent Newcastle disease virus field isolates from the southeastern United States and Puerto Rico. Avian Dis, 40, 382-390, 1996.
11. Naresh J, Yogesh C, Ashok K C, Marthade A, Patrick TR, Sagar MG: Phylogenetic analysis of Newcastle disease viruses isolated from waterfowl in the Upper Midwest Region of the United States. J Virol, 81, 12641-12653, 2007.
12. Liu XW, Wang XQ, Wu S, Hu SL, Peng Y, Xue F, Liu XF: Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata ) at live bird markets in Eastern China and characterization of the viruses isolated. Avian Pathol, 38, 377-391, 2009.
13. Lee EK, Jeon WJ, Kwon JH, Yang CB, Choi KS: Molecular epid emiological investigation of Newcastle disease virus from domestic ducks in Korea. Vet Microbiol, 134, 241-248, 2009.
14. Liu H, Wang Z, Wang Y, Sun C, Zheng D, Wu Y: Characterization of Newcastle disease virus isolated from waterfowl in China. Avian Dis, 52, 150-155, 2008.
15. Zhang SP, Wang XT, Zhao CG, Liu DH, Hu YX, Zhao JX, Zhang GZ: Phylogenetic and pathotypical analysis of two virulent Newcastle Disease Viruses isolated from domestic ducks in China. PLos One , 6, e25000, 2011.
16. Abolnik C, Horner RF, Bisschop SP, Parker ME, Romito M, Viljoen GJ: Aphylogenetic study of South African Newcastle disease virus strains isolated between 1990 and 2002 suggests epidemiological origins in the Far East. Arch Virol, 149, 603-619, 2004.
17. Czegledi A, Herczeg J, Hadjiev G, Doumanova L, Wehmann E, Lomniczi B: The occurrence of five major Newcastle disease virus genotypes (II, IV, V, VI an d VIIb) in Bulgaria between 1959 and 1996. Epidemiol Infect, 129, 679-688, 2002.
18. Alexander DJ: Gordon Memorial Lecture: Newcastle disease. Br Poult Sci, 42, 5-22, 2001.
19. Liang R, Cao DJ, Li JQ, Chen J, Guo X, Zhuang FF, Duan MX: Newcastle disease outbreaks in western China were caused by the genotypes VIIa and VIII. Vet Microbiol, 87 (3): 193-203, 2002.
20. Zanetti F, Berinstein A, Carrillo E: Efect of host selective pressure on Newcastle disease virus virulence. Microbial Pathog, 44, 135-140, 2008.
21. King DJ, Seal BS: Biological and molecular characterization of Newcastle disease virus isolates from surveillance of live bird markets in the northeastern United States. Avian Dis, 41, 683-689, 1997.
22. Hua BX, Huang YY, He YF, Xu CT, Lu XS, Zhang W, Meng B, YanSG, Zhang XM: Avian infuenza virus and Newcastle disease virus (NDV) surveillance in commercial breeding farm in China and the characterization of Class I NDV isolates. Vet Microbiol, 144, 82-86, 2010.
23. Kouji S, Genki S, Orie T, Yuko W, Alam J, Masayuki N, Kazuaki T: Characterization of Newcastle disease virus isolated from Northern Pintail (Anasacuta) in Japan. J Vet Med Sci, 69, 1307-1311, 2007.
24. Jorgensen PH, Handberg KJ, Ahrens P, Therkildsen OR, Manvell RJ, Alexander DJ: Strains of avian paramyxovirus type 1 of low pathogenicity for chickens isolated from poultry and wild birds in Denmark. Vet Rec, 154, 497-500, 2004.
25. Collins MS, Bashiruddin JB, Alexander DJ: Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol, 128, 363-370, 1993.
26. Huang Y, Wan HQ, Liu HQ, Wu YT, Liu XF: Genomic sequence of an isolate of Newcastle disease virus isolated from an outbreak in geese: A novel six nucleotide insertion in the non-coding region of the nucleoprotein gene. Arch Virol, 149, 1445-1457, 2004.
27. Lomniczi B, Wehmann E, Herczeg J, Ballagi-Pordány A, Kaleta EF, Werner O, Meulemans G, Jorgensen PH, Manté AP, Gielkens AL, Capua I, Damoser J: Newcastle disease outbreaks in recent years in Western Europe were caused by an old (VI) and a novel genotype (VII). Arch Virol, 143, 49-64, 1998.
28. Muhammad M, Muhammad A, Muhammad TK, Siamak Z, Mikael B: Genomic and biological characterization of a velogenic Newcastle disease virus isolated from a healthy backyard poultry fock in 2010. J Virol, 9, 46, 2012.
29. Yang CY, Shieh HK, Lin YL, Chang PC: Newcastle disease virus isolated from recent outbreak s in Taiwan phylogenetically related to viruses (genotype VII) from recent outbreaks in western Europe. Avian Dis, 43 , 125-130, 1999.