Gebe ve gebe olmayan sıçanlarda karbontetraklorürün (CCI4) bazı biyokimyasal değerler üzerine etkileri

Bu araştırmada, CCl4’ün gebe ve gebe olmayan sıçanlarda bazı biyokimyasal değerleri üzerine etkisinin ortaya konması amaçlanmıştır. Araştırmada 150-250 g, 6-8 aylık, 6 gebe ve 6 gebe olmayan 12 Wistar Albino sıçan kullanıldı. Sıçanlar rasgele iki gruba ayrılarak altı sıçandan oluşan bir grubun kafesine fertil bir erkek sıçan bırakılarak dişilerin gebe kalması sağlandı. Gebeliğin birinci haftasındaki ve gebe olmayan sıçanların kalplerinden hafif eter anestezisi altında deneme öncesi biyokimyasal muayeneler için kan alındı. Akut intoksikasyon oluşturmak için sıçanlara intraperitonal olarak tek doz halinde zeytinyağı ile 1/1 süspansiyonu hazırlanan CCl4 (2 ml/kg) enjekte edildikten sonra 24. saatte kan alınarak bazı biyokimyasal değerler belirlendi. CCl4 denemesi sonrası gebe ve gebe olmayan sıçanların karaciğer enzimlerinden aspartat amino transferaz (AST), alanin amino transferaz (ALT) ve laktat dehidrojenaz (LDH) artışın saptanması, karaciğer hücrelerinde, önemli ölçüde hasar oluşturduğu sonucunu göstermiştir.

Anahtar Kelimeler:

karaciğer, sıçan, karbon tetraklorür, gebelik

Effects of carbontetrachloride (CCI4) on some blood ualues in pregnant and non-pregnant rats

In this investigation, the effect of CCl4 on some biochemical values in pregnant and non-pregnant rats was aimed to study. Twelve female 6-8 months old Wistar Albino rats weighing 150-250 g were used. Rats were divided randomly into two groups containing six in each. In one group pregnancy was achived by housing six female rats with a fertile male for one night. Blood was withdrawn from ether anesthetized rats through heart puncture, on the seventh day of pregnancy and non-pregnant rats for some biochemical measurements. Acute intoxication was induced in rats by injection a single dose of a mixture of CCl4 + olive oil (1:1), 2 ml/kg, intraperitonally. Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH) enzyme activities were increased significantly in pregnant and non-pregnant rats after CCl4 treatment. These results indicate the hepatic damage caused by acute toxic effects of carbon tetrachloride both in pregnant and non-pregnant rats.

Keywords:

liver, rats, carbon tetrachloride, pregnancy,

PDF

___

ÇCnar A, Yörük M, Meral İ, KClCçalp D, Koç A, Ertekin A: Karbon tetraklorür (CCl4) ile tavşanlarda deneysel olarak oluşturulan akut ve kronik intoksikasyonun karaciğerin histolojik yapTsTna, bazT hematolojik değerlere ve elektrokardiyogram üzerine etkileri. Turk J Vet Anim Sci, 23, 235-242, 1999.
Maianskii DN, Kutina SN: Characteristics of the development of acute hepatitis in rats stimulated with prodigiozan. Biul Eks Biol Med, 87, 622-624, 1979.
Kaneko T, Wang Pei-Yu, Sato A: Relationship between blood/air partition coefficients of lipophilic organic solvent and blood triglyceride levels. Toxicology, 143, 203-208, 2000.
Adams HR: Veterinary Pharmacology and Therapeuties. 7th Edition. Iowa State University Press/AMES, 1995.
Kaya S, Pirinçci İ, Bilgili, A: Veteriner Uygulamalı Farmakoloji. Cilt 2, Baskı 2, Medisan Yayınevi – Ankara, s: 435-436, 2000.
Shimizu H, Uetsuka K, Nakayama H, Doi K: Carbon tetracholoride-induced acute liver injury in mini and wistar rats. Exp Toxica Pathol, 53,11-17, 2001.
Murakami T, Nagamura Y, Hirano K: The effect of ethanolamine on acute carbon tetrachloride intoxication. Biol Pharm Bull, 21, 84-86, 1998.
Nokata M, Katoh M, SugCmoto T: Protective effect of melatilate (diisopropyl 1,3-dithiol-2-yli denemelanate) on carbon tetrachloride-induced liver injury in mice and rats. J Toxicol Sci, 10: 279-288, 1985.
Ladics, GS, Smith C, Elliott GS, Slone TW, Loveless SE: Further evaluation of the incorporation of an immunotoxicological functional assay for assesing humoral immunity for hazard identification purposes in rats in a standart toxicology study. Toxicology, 126, 137-152,1998.
Murakami T, Nagamura Y, Hirano K: Ethanolamine stimulates repair processes in acute CCl4 damage of mouse liver. Toxicol Lett, 94, 137-144, 1998.
Muriel P: Nitric oxide protection of rat liver from lipid peroxidation, collagen accumulation, and liver damage induced by carbon tetrachloride. Biochemical Pharm, 56, 773-779, 1998.
Clayton GD, Clayton FE: Patty’s Industrial Hygiene and Toxicology. 3rd Revised Edition. Vol.: 2B, Toxicology. A Wiley – Interscience Publication, 1981.
Imaizumi Y, Sugimoto T, Kasai T: Effect of NKK-105 on fatty liver induced by carbon tetrachloride. Japan J Pharmacol, 31, 15-21, 1981.
Ema M, Harazono A, Hirose A, Kamata E: Protective effects of progesterone on implantation failure induced by dibutyltin dichloride in rats. Toxicol Lett, 143, 233-238,2003.
Singh J, Kaur AH, Mathur SK: Protection of CCl4-induced liver damage in rats by some calcium channel blockers. Indian J Physiol Pharm, 39, 275-278, 1995.
Kaneko JJ, Harvey JW, Bruss ML: Clinical Biochemistry of Domestic Animals. Academic Press, London. pp: 895-899, 905, 1997.
Matsuzawa T, Nomura N, Unno T: Clinical pathology reference ranges of laboratory animals. J Vet Med Sci, 55, 351-362, 1993.
Nishie K: Comparison of the effects of Nnitrosodimethylamine on pregnant and nonpregnant Holtzman rats. Food and Chemical Toxicol, 21, 453-462,1983.
Waynforth HB: Experimental and Surgical Techniques in the Rat. Academic Press, London, pp: 239-245, 1980.
Deo MG, Roy H, Ramalingaswami V: Protein deficiency in carbon tetrachloride-induced hepatic lesions. Arch Pathol, 99, 147-151, 1975.
Farombi EO: Mechanisms for the hepatoprotective action of kolaviron: Studies on hepatic enzymes, microsomal lipids and lipid peroxidation in carbontetrachloridetreated rats. Pharm Res, 42, 75-80, 2000.
Munilla MA, Herrera E: Maternal hypertriglyceridemia during late pregnancy does not affect the increase in circulating triglycerides caused by the long term consumption of a sucrose rich diet by rats. J Nutr, 130, 2883-2888,2000.
Vadiraja BB, Gaikwad NW, Madyastha KM: Hepatoprotective effect of C-phycocyanin: Protection for carbon tetracholoride and R-(+)-pulegone-mediated hepatotoxicty in rats. Bioc Biop Res Commun, 249, 428-431,1998.