Atia BASHEER, Mehwish SHAREEF, Mahjabeen ISLAM, Imran ZAHOOR

Beetal Keçisinde FecXG Polimorfizmi ve Filogenetik İlişkisinin İncelenmesi

Bu çalışmanın amacı Beetal keçisinde BMP-15 geninin ekzon 2’sinde 141 bp fragmanında FecXG mutasyonunun insidansını ve diğer keçilerle olan filogenetik ilişkisini belirlemektir. Çalışmada 60 Beetal keçisi rastgele seçildi ve vücut ağırlıklarına göre eşit iki gruba ayrıldı. BMP-15 geninin ekzon 2’sindeki polimorfizmi araştırmak amacıyla PCR-RFLP ve DNA sekanslama teknikleri kullanıldı. Tüm hayvanlarda, Hinf1 ile kesilme sonrasında elde edilen saf ürün iki bantlı (111bp ve 54bp) heterozigot taşıyıcılar olarak gözlemlendi. Beetal keçilerinde FecXG polimorfizm analizi, büyüme oranlarındaki farkın oluşmasından sorumlu olan genetik faktörlerin BMP-15 geninin mutasyonlu alleli ile ilgili olmadığını gösterdi. Bu nedenle, BMP-15 geninin diğer bölgelerindeki polimorfizmin, Beetal keçilerinde büyüme oranlarındaki farklılığının oluşmasından sorumlu olabileceği düşüncesi ile araştırılması önerilmiştir. BMP-15 geninin mRNA’sının filogenetik ağacı oluşturuldu ve elde edilen sonuçlar Teddy’nin dışgrup olduğunu ortaya koymuştur. Ayrıca, bu çalışmada kullanılan tüm ırklar arasında, Teddy daha küçük boyutu ve daha fazla üretkenliği nedeniyle diğer ırklardan fenotipik olarak farklıydı. Ancak, geri kalan tüm ırklar tek bir büyük kuşakta bulunurken Siyah Bengal ve Markhoz keçisi başka bir alt kuşakta yer aldı ve ortak atadan azami sapma gösterdiler. Beetal, Siyah Bengal ve Markhozdan sonra ortak ataları ile birlikte tek kuşakta yer almaktadır.

Study of FecXG Polymorphism in Beetal Goat and Its Phylogenetic Relationship

The objective of the current study was to detect the incidence of FecXGmutation in 141bp fragment of exon 2 of BMP-15 gene in Beetalgoat and to determine its phylogenetic relationship with other goat breeds. In this study 60 Beetal goats were randomly selected anddivided equally into two groups on the basis of their body weights. The PCR-RFLP and DNA sequencing techniques were employed toexplore polymorphism in exon 2 of BMP-15 gene. Upon digestion with Hinf1, the purified product showed heterozygous carriers withtwo bands (111bp, and 54bp) in all animals. The analysis of polymorphism for FecXG in Beetal goat indicates that the genetic factorresponsible for difference in growth rates is not related to the reported mutated alleles of BMP-15 gene. Therefore, it is suggested thatpolymorphisms in the other regions of BMP-15 gene should be explored which might be responsible for the difference of growth ratesin Beetal goat. Phylogenetic tree of mRNA of BMP-15 gene was constructed and the results revealed Teddy as outgroup. Moreover,among all the breeds used in this analysis Teddy is also phenotypically different from other due to its smaller size and greater prolificacy.However, all the remaining breeds were present in a single large clade, with Black Bengal and Markhoz goat in a further single sub-cladeand showing maximum divergence from the common ancestor. The Beetal was next to Black Bengal and Markhoz and along with theircommon ancestor they were present in a single clade.

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