Ayşe EKEN, Burcu ÜNLÜ-ENDİRLİK, Elçin BAKIR, AYŞE BALDEMİR KILIÇ, Arzu Hanım YAY, FAZİLE CANTÜRK TAN

Effect of Laurocerasus officinalis Roem. (Cherry Laurel) Fruit on Dimethoate Induced Hepatotoxicity in Rats

Dimetoat en önemli organofosfatlı insektisitlerden biridir, serbest radikallerin üretimine ve antioksidanlarda değişimlere yol açarak oksidatif strese neden olur. Çalışmanın amacı, ratlarda antioksidan bileşikler içeren Laurocerasus officinalis Roem. meyve ekstresinin dimetoat kaynaklı karaciğer toksisitesindeki koruyucu etkilerini incelemektir. Ratlar aşağıdaki gibi altı gruba ayrıldı: Kontrol grubu; dimetoate ile muamele edilen grup; L. officinalis ile muamele edilen grup; dimetoat öncesi L. officinalis verilen grup; dimetoat öncesi vitamin C verilen grup; dimetoat sonrası L. officinalis ile muamele edilen grup. Karaciğer fonksiyon testleri olarak aspartat transaminaz (AST), alanin transaminaz (ALT) ve toplam bilirubin (TBil) ile toplam oksidan durum (TOS), toplam antioksidan durum (TAS), süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve malondialdehit (MDA) gibi oksidatif stres parametreleri ölçüldü. DNA hasarı komet testi ile belirlendi. Sonuçlar, dimetoat'ın kontrol grubuna kıyasla AST, ALT, TOS, MDA, DNA hasarında belirgin bir artışa ve TAS, SOD, CAT, GPx'de önemli bir azalmaya neden olduğunu gösterdi. Bununla birlikte, dimetoat'a maruz bırakılan ratlara L. officinalis veya vitamin C'nin uygulanması, bu biyokimyasal ve oksidatif stres parametrelerini neredeyse normal seviyelere getirdi. Sonuç olarak, dimetoat'ın rat karaciğeri üzerindeki toksik etkileri esas olarak hepatik fonksiyon enzimleri, oksidatif stres ve DNA hasarına atfedilirken, bu etkiler L. officinalis meyve ekstresi tarafından büyük ölçüde iyileştirilmiştir

Sıçanlarda Dimetoatla Oluşturulan Hepatotoksisite Üzerine Taflan (Laurocerasus officinalis Roem.) Meyvesinin Koruyucu Etkisi

Dimethoate is one of the most important organophosphate insecticides and may cause oxidative stress leading to production of free radicals and alterations in antioxidants. The aim of this study was to investigate the protective effects of Laurocerasus officinalis Roem. fruit extract containing antioxidant compounds on dimethoate-induced hepatotoxicity in rats. The rats were divided into six groups as follows: Control group; dimethoate-treated group; L. officinalis-treated group; the group of pre-treatment with L. officinalis prior to dimethoate; the group of pre-treatment with vitamin C before dimethoate; the group of post-treatment with L. officinalis after dimethoate. Aspartate transaminase (AST), alanine transaminase (ALT), and total bilirubin (TBil) as liver function tests and the oxidative stress parameters such as total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were measured. The DNA damage was determined with comet assay. The results indicated that dimethoate caused a significant increase in AST, ALT, TOS, MDA, DNA damage and an important decrease in TAS, SOD, CAT, GPx as compared to control group. However, administration of L. officinalis or vitamin C to dimethoate-exposed rats restored these biochemical and oxidative stress parameters to nearly normal levels. In conclusion, toxic effects of dimethoate on rat liver are mainly attributed to hepatic function enzymes, oxidative stress and DNA damage, while these effects were largely ameliorated by L. officinalis fruit extract

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