ÇİĞDEM ÇEBİ ŞEN, Ricardo FAUNDEZ, Piotr JURKA, ERGÜN AKÇAY, Monika GOLOBOW ETRAJTIS, Pınar AMBARCIOĞLU

Evaluation of the Canine Epididymal Sperm Morphology with two Different Staining Methods, One Fixative Solution and Motile Sperm Organelle Morphology Examination (MSOME)

Bu çalışmanın amacı, taze köpek sperması örneklerinde spermatozoonların morfolojik özellikleri üzerine Motil Sperm Organel Morfolojisi Muayenesi (MSOME), bir fiksatif solüsyon ve iki farklı boyanın kullanımını ve etkinliğini karşılaştırmaktı. Kastrasyon işleminden sonra, 20 adet köpek testisinden kauda epididimisler elde edildi. Epididimal spermada morfolojik bozukluklar, Hancock sıvı fikzasyon yöntemi, aniline blue boyama yöntemi, Diff Quik boyama yöntemi ve MSOME tekniği ile değerlendirildi. Her bir köpekten bir örnek alınarak, konvensiyonel semen analizi ve MSOME analizi aynı örnekte eş zamanlı olarak incelendi. Morfolojik olarak ortalama abnormal baş ve akrozom defekti MSOME tekniğine göre diğer üç farklı methodta önemli derecede yüksek bulunmuştur. MSOME tekniği ile 3 farklı yöntem karşılaştırıldığında, anilin boyama yönteminde baş bozukluk oranı bulunan anormal spermatozoa oranı önemli derecede yüksek bulunurken, köpek spermatozoa morfolojisi değerlendirilmesinde Aniline blue boyama yönteminin etkili bir yöntem olduğu sonucuna varıldı. Spermatozoa morfolojisinin değerlendirilmesinde üç farklı method ile MSOME tekniği arasında herhangi bir paralellik bulunmadı.

Motil Sperm Organel Morfolojisi Muayenesi (MSOME) ve Fiksatif Solüsyon, İki Farklı Boyama Tekniği İle Köpek Epididimal Spermatozoa Morfolojisinin Değerlendirilmesi

The aim of this study was to compare the use and effectiveness of two different stains, one fixative solution and Motile Sperm Organelle Morphology Examination (MSOME) on morphological characteristics of spermatozoa in fresh dog semen samples from epididymis. After routine castration, cauda epididymides were collected from 20 dog testes. Morphological abnormalities were evaluated by using Hancock's buffered formol saline solution, the aniline blue stain, Diff-Quik stain and MSOME analysis in epididymal semen. Conventional semen analysies and MSOME were simultaneously performed on the same sample from each dog. Percentage of abnormal spermatozoa, head abnormality and acrosomal defects were significantly higher in samples with two different staining methods and one fixative solution than MSOME technique. It was concluded that the usage of the Aniline blue stain may be an efficient method for evaluating the sperm morphology of dog semen. Although no correlation was established amoung four different methods on sperm morphology assessment.

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