Seviye YEŞİL LAZERİN ETKİSİ, Soudeh ANSARI, Pejman MORTAZAVI

The Effect of Low Level Green LASER on Autologous Full-Thickness Free Skin Graft in Rats

Tam katmanlı otolog deri grefti üzerine yeşil LED lazerin etkisi sıçanlarda test edildi. On adet yetişkin erkek Wistar sıçan düşük seviye yeşil LED lazer radyasyon grubu ve kontrol grubu olmak üzere iki gruba ayrıldı. Tüm kalınlıkta kenarları 3 cm olan bir kare insizyon hayvanların dorsal kısmında oluşturuldu. Sonrasında, deri uzaklaştırıldı ve 90 derece döndürülerek yerine tekrar dikildi. Yeşil LED radyasyonu (1 J/cm2, 60 s, ve 532 nm) operasyon sonrası 6 gün boyunca her gün test grubuna uygulandı. Biyopsiler cerrahi işlemin 3, 5, 7 ve 14. günlerde alınarak patoloji laboratuarına gönderildi. Greftin boyutu aynı günlerde kaydedildi. Sonuçlar lazerin cerrahi müdahale bölgesinde doku otogreftini sağladığını gösterdi. Makroskopik ve mikroskopik çalışmalar kontrol grubunda greft dokusunun anlamlı derecede (P>0.05) çekme ve fibrozise uğradığını ortaya koydu

Sıçanlarda Tam-Katmanlı Otolog Serbest Deri Greftine Düşük Seviye Yeşil LAZER'in Etkisi

The effect of green LED laser on free full-thickness autologous skin graft was tested in rats. Ten adult male Wistar rats were divided into two groups of in each group as test (Low level green LED laser irradiation) and the control group. A full-thickness square incision with the sides of three centimeters was created at the dorsal region of animals. Then, the skin was removed and replaced with a rotation of 90 degrees and sutured. Green LED irradiation (1 J/cm2, 60 s, and 532 nm) was performed immediately in the test group daily over a period of 6 days post-operatively. Biopsy taking was carried out on 3, 5, 7 and 14 days after surgery and was sent to the pathology laboratory. The size of graft was recorded on determined days. The results showed that the laser with the amount of has ability to maintain tissue autograft in the surgical site. Macroscopic and microscopic studies showed that the graft tissue in the control group showed significantly (P>0.05) shrinkage and fibrosis.

PDF

___

Swaim SF: Skin graft. In, Slatter D (Ed): Textbook of Small Animal Surgery. 3rd ed., Vol. 1. 321-338, Saunders, Philadelphia, 2003.
Pavletic MM: Atlas of Small Animal Wound Management and Reconstructive Surgery. 3rd ed., 404-430, Wiley-Blackwell, London, 2010.
Fossum TW, Dewey CW, Horn CV, Johnson AL, MacPhail CM, Radlinsky MG, Schulz KS, Willard MD: Small Animal Surgery. 4th ed., 256, Elsevier Mosby, St. Louis, 2013.
Bohling MW, Swaim SF: Skin grafts. In, Tobias KM, Johnston SA (Eds): Veterinary Surgery Small Animal. Vol. 2. 1270-1290, Elsevier Saunders, Missouri, 2012.
Fekrazad R, Chiniforush N, Bouraima SA, Valipour M, Aslani M, Zare M, Ashtiani Safari O: Low level laser therapy in management of complications after intra oral surgeries. J Lasers Med Sci, 3 (4): 135-140,
Gal P, Kilik R, Mokry M, Vidinsky B, Vasilenko T, Mozes S, Bobrov N, Tomori Z, Bober J, Lenhardt L: Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: Standardization of semi-quantitative and quantitative histological assessments. Vet Med, (12): 652-659, 2008.
Machneva TV, Protopopov DM, Vladimirov IuA, Osipov AN: A study of the effect of low-intensity laser radiation of the blue, green, and red spectral regions on the healing of experimental skin wounds in rats. Biofizika, 53 (5): 894-901, 2008.
Rodrigo SM, Cunha A, Pozza DH, Blaya DS, Moraes JF, Weber JB, de Oliveira MG: Analysis of the systemic effect of red and infrared laser therapy on wound repair. Photomed Laser Surg, 27, 929-935, 2009. DOI: 1089/pho.2008.2306
Walsh LJ, Trinchieri G, Waldorf HA, Whitaker D, Murphy GF: Human dermal mast cells contain and release tumor necrosis factor-which induces endothelial leukocyte adhesion molecule-1. Proc Natl Acad Sci, , 4220-4224, 1991. DOI: 10.1073/pnas.88.10.4220
Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD: Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med, 36, 8-12, 2005. DOI: 10.1002/lsm.20117
Nasirian N, Nasirian A: Effect of low level laser therapy on the surgical wound healing in hamster. IJP, 7 (4): 236-240, 2012.
Fattahian HR, Nasirian A, Mortazavi P: The role of red and infrared low level laser therapy on unmeshed full-thickness free skin autograft in rabbits: As an animal model. Kafkas Univ Vet Fak Derg, 19, 829-836, 2013. DOI: 10.9775/kvfd.2013.8902