Evaluation of Motility Hormones in Dairy Cattle with Omasal Impaction and Caecal Dilatation

Bu çalışmanın amacı, omazum konstipasyonlarında (OK) ve sekum dilatasyonunda (SD) sığırlarda motilite hormon düzeylerini değerlendirmektir. Çalışmada 4 OK, 4 SD (volvulus olmaksızın) ve 10 sağlıklı kontrol sığır kullanıldı. Serum ghrelin, motilin, gastrin ve leptin konsantrasyonları ELISA kullanılarak belirlendi. Serum alanine aminotrasferaz (ALT), aspartate aminotrasferaz (AST), gama glutamil transferaz (GGT) aktiviteleri ve sodyum (Na), potasyum (K) ve klor (Cl) konsantrasyonları spektrofotometre kullanılarak ölçüldü. Serum ALT, AST ve GGT aktiviteleri OK'lı sığırlarda yüksekti. Serum Cl konsantrasyonu ise OK'lu sığırlarda CD'lu sığırlardan düşüktü. OK'lı sığırlarda yüksek gastrin ve motilin seviyesi ve SD'lı sığırlarda düşük leptin seviyesi tespit edildi. Sonuç olarak, bu çalışma omazum konstipasyonu ve sekum dilatasyonlu sığırlarda GI motilite hormone agonistlerinin kullanımını desteklememektedir. Leptin negative enerji dengesini belirleyebilir

Sekum Dilatasyonlu ve Omazum Konstipasyonlu Sütçü Sığırlarda Motilite Hormonlarının Değerlendirilmesi

The aim of this study was to evaluate the motility hormones levels in cattle with caecal dilatation (CD) and omasal impaction (OI). In this study, four cows with OI, four cows with CD (without volvulus) and ten healthy controls were used. Serum ghrelin, motilin, gastrin and leptin concentrations were determined using ELISA. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT) activities and sodium (Na), potassium (K) and chloride (Cl) concentrations were measured using a spectrophotometer. Serum ALT, AST and GGT activities were higher in OI cows. Serum Cl concentrations were lower in OI cows than in CD cows. Higher gastrin and motilin levels in OI and lower leptin levels in CD cows were found. In conclusion, this study dose not supports the use of GI motility hormones agonists in cows suffering from omasal impaction and caecal dilatation. Leptin might be indicated in negative energy balance

PDF

___

Inui A, Asakawa A, Bowers CY, Mantovani G, Laviano A, Meguid MM, Fujmiya M: Ghrelin, appetite, and gastric motility: The emerging role of the stomach as an endocrine organ. FASEB J,18, 439-456, 2004. DOI: 10.1096/fj.03-0641rev
Radostits OM, Gay CC, Hinchcliff KW, Constable PD: Impaction of the omasum. Diseases of the intestines of ruminants. In, Rodastits OM, Gay CC, Hinchcliff KW, Constable PD (Eds): Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats. 10th ed., 352-353, 376, Elsevier Health Sciences, Philadelphia, 2007.
Knowlton KF, Nelson JM: World of Dairy Cattle Nutrition. Holstein Foundation. pp.7-8, 1989.
Divers TJ, Peek SF: Rebhun's Diseases of Dairy Cattle. 2nd ed., p.184, Elsevier Health Sciences, ISBN-13: 978-1-4160-3137-62008.
Turkar S, Uppal SK: Blood biochemical and ruminal liquor profile in buffaloes (Bubalus bubalis) showing omasal impaction. Vet Res Commun, , 967-975, 2007. DOI: 10.1007/s11259-007-0027-2
Umakanthan T: Native drug therapy for omasal impaction in cattle. Indian Vet J, 79 (3): 295-296, 2002.
El-Attar HM, Ghanem MM, Abd El-Raof YM: Clinical, biochemical and haematological studies on omasal impaction in cows: The 2nd Scientific Conference, Faculty of Veterinary Medicine, Benha University, Egypt, January, 2007.
Hussain SA, Uppal SK, Randhawa C, Sood NK, Mahajan SK: Clinical characteristics, hematology, and biochemical analytes of primary omasal impaction in bovines. Turk J Vet Anim Sci, 37, 329-336, 2013. DOI: 10.3906/ vet-1205-31
Gerloff BJ, Herdt TH, Emery RS: Relationship of hepatic lipidosis to health and performance in dairy cattle. J Am Vet Med Assoc, 188(8): 845- , 1986.
Smith BP (Ed): Large Animal Internal Medicine. Fifth ed., Chapter 22: Gamma Glutamyltransferase (GGT). p.367, Elsevier Publishing, USA. 2015.
Braun U, Beckmann C, Gerspach C, Hässig M, Muggli E, Knubben- Schweizer G, Nuss K: Clinical findings and treatment in cattle with caecal dilatation. BMC Vet Res, 8, 1-9, 2012. DOI: 10.1186/1746-6148-8-75
Ohno T, Mochıkı E, Kuwano H: The roles of motilin and ghrelin in gastrointestinal motility. Int J Pept, Article ID 820794, 6 pages. 2010. DOI: 1155/2010/820794
Koenig JB, Cote N, LaMarre J, Harris WH, Trout DR, Kenney DG, Monteith G: Binding of radiolabeled porcine motilin and erythromycin lactobionate to smooth muscle membranes in various segments of the equine gastrointestinal tract. Am J Vet Res, 63, 1545-1550, 2002. DOI: 10.2460/ajvr.2002.63.1545
Wertz A, Knight T, Kreuder A, Bohan M, Beitz D, Trenkle AH: Effect of feed intake on plasma ghrelin concentration in beef cattle. Animal Industry Report: AS 650, ASL R1881. http://lib.dr.iastate.edu/ans_air/ vol650/iss1/40, 2004. Accessed: 02.12.2014.
Bunnett NW: The localization of gastrin-like immunoreactivity (GIRL) in the alimentary tract of the sheep. Q J Exp Physiol, 69, 521-529, 1984.
Wang W, Cheng L, Guo J, Ma Y, Li F: Expression of Ghrelin in gastrointestinal tract and the effect of early weaning on Ghrelin expression in lambs. Mol Biol Rep, 41, 909-914, 2014. DOI: 10.1007/ s11033-013-2935-2
Sen I, Ok M, Turgut K, Birdane FM, Guzelbektas H: Role of gastrin in the aetiology of abomasal displacement in dairy cows. Vet Rec, 151, 26-27, DOI: 10.1136/vr.151.1.26
Ozturk AS, Guzel M, Askar TK, Aytekin I: Evaluation of the hormones responsible for the gastro-intestinal motility in cattle with displacement of the abomasum: Ghrelin, motilin and gastrin. Vet Rec, 172, 636, 2013. DOI: 10.1136/vr.101322
Chan JL, Bullen J, Lee JH, Yiannakouris N, Mantzoros CS: Ghrelin levels are not regulated by recombinant leptin administration and/or three days of fasting in healthy subjects. J Clin Endocrinol Metab, 89, 335- , 2004. DOI: 10.1210/jc.2003-031412
Iwasaki E, Suzuki H, Masaoka T, Nishizawa T, Hosoda H, Kangawa K, Hibi T: Enhanced gastric ghrelin production and secretion in rats with gastric outlet obstruction. Digest Dis Sci, 57, 858-864, 2012. DOI: 10.1007/ s10620-011-1953-y
Block SS, Butler WR, Ehrhardt RA, Bell AW, Van Amburgh ME, Boisclair YR: Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol, 171, 339- , 2001. DOI: 10.1677/joe.0.1710339