Th1/Th2 Cytokine Balance and SOCS3 Levels of Female Offspring Born from Rats with Gestational Diabetes Mellitus [1]

Gebelikte şekillenen diabetes mellitus, hem anne sağlığı hem de yavru sağlığı için son derece önemli ve giderek görülme sıklığı artan bir metabolik bozukluktur. Bu hastalığın gelişimi ve tedavisine yönelik yeni araştırmalara ihtiyaç vardır. Bu çalışmada da ileri gebe ratlarda farklı dozlarda streptozotosinle oluşturulan diabetes mellitusun yavruların, kan serumlarındaki Th1/Th2 sitokin dengesi ve SOCS3 düzeyleri üzerine etkisinin belirlenmesi amaçlanmıştır. Bu amaçla, 21 adet ileri gebe rat kullanıldı. Ratlar rastgele üç gruba ayrılarak 1. gruptaki hayvanlar kontrol grubu olarak ayrıldı. Streptozotosin 2. gruptaki hayvanlara 40 mg/kg, 3. gruptaki hayvanlara 60 mg/kg dozda intraperitonal olarak uygulandı. Daha sonra hayvanların doğumları takip edildi. Yavrular 1 aylık olduktan sonra her hayvanın 1 dişi yavrusu dekapite edildi. Dekapite edilen hayvanların kanları alındı ve kan serumlarında Th1 [Interferon gamma (IFN?), Interleukin 2 (IL-2), Tumor necrosis factor alpha (TNF?)], Th2 [Interleukin 4 (IL-4), Interleukin 5 (IL-5), Interleukin 10 (IL-10)] sitokin düzeyleri xMAP® tespit teknolojisini esas alan multiplex immünoassay ve Suppressor of cytokine signalling 3 (SOCS3) düzeyleri ise ticari ELİSA kiti ölçüldü. Sonuç olarak, grup 1'deki IFN-? (1,59±0,28 pg/ml) değerinin diğer gruplara nazaran daha düşük olduğu, grup 2 ve 3'teki TNF-? (2,24±0,20, 2,21±0,19 pg/ml) değerlerinin de grup 1'den fazla olduğu, tüm gruplardaki IL-4, IL-6, IL-10 ve SOCS3 konsantrasyonlarının karşılaştırılması sonucunda ise gruplar arasında istatistiki anlamda bir fark olmadığı belirlendi. Sonuç olarak; Th1/Th2 sitokin dengesinin Th1'e doğru kaydığı gözlendi. Bu da hücresel immünitenin daha ön planda olduğunu göstermektedir

Gebelikte Diabetes Mellitus Şekillenmiş Ratlardan Doğan Dişi Yavrularda Th1/Th2 Sitokin Dengesi Ve SOCS3 Düzeyleri

Diabetes mellitus during pregnancy is a metabolic disorder that is very important in regard to both the health of the mother and the baby. We determine the effect of diabetes mellitus generated by streptozotocin with different dosages in pregnant rats on serum Th1/Th2 cytokine balance and SOCS3 levels. Twenty-one pregnant rats in their late pregnancy were used for this purpose. The rats were divided into three groups randomly and the rats in the first group were used as the control group. Streptozotocin was administered intraperitoneally at a dosage of 40 mg/kg to the rats in the second group and at a dosage of 60 mg/kg to the rats in the third group. One female offspring of each rat was decapitated and the blood of the decapitated rats was collected and Th1 [Interferon gamma (IFN?), Interleukin 2 (IL-2), Tumor necrosis factor alpha (TNF?)], Th2 [Interleukin 4 (IL-4), Interleukin 5 (IL-5), Interleukin 10 (IL-10)] cytokine levels were measured using a multiplex immunoassay based on xMAP® detection technology and the suppressor of cytokine signaling 3 (SOCS3) levels were measured using a commercial ELISA kit. The IFN-? (1.59±0.28 pg/mL) levels in group 1 were lower than those in the other groups. The TNF-? (2.24±0.20, 2.21±0.19 pg/mL) levels in groups 2 and 3 were higher than in group 1, and the IL-4, IL-6, IL-10 and SOCS3 concentrations were not significantly different among groups. The SOCS3 levels of the offspring were not different among the groups, and the IFN-? and TNF-? blood concentrations were increased; the Th1/Th2 cytokine balance was shifted toward Th1. We have observed that the SOCS3 levels of offspring born from mothers generated to be diabetic by administration of streptozotocin in late pregnancy were not different among various groups, and the IFN-? and TNF-? blood concentration were increased. As a result, the Th1/Th2 cytokine balance was shifted toward Th1. This suggests that more prominent cellular immunity

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