Association of Single Nucleotide Polymorphism in Bone Morphogenetic Protein Receptor 1B (BMPR-1B) Gene with Growth Traits in Chicken

Büyüme özellikleri çok sayıda genin kontrolü altındadır. İlgili genlerdeki genetik bilgiyi anlamak, marker-destekli seleksiyon yoluyla seçim ve üreme sürecinde yardımcı olur. Bu çalışmanın amacı, tavuklarda BMPR-1B genindeki A287G SNP ile büyüme özellikleri arasındaki ilişkiyi araştırmaktır. PCR-RFLP tekniği kullanılarak, 240 bireyde bir tek nükleotid polimorfizmi tespit edildi ve sekanslama ile doğrulandı. Allellik ve genotipik frekanslar Ki-kare testi kullanılarak karşılaştırıldı. Her polimorfizm genotipi ve özellikler arasındaki ilişkiler SAS istatistiksel yazılımının Genel Lineer Modeli kullanılarak analiz edildi. Fayoumi ve Rhold Adası Kırmızı tavuklarında üç genotipi (AA, AG ve GG) saptandı. Sıralama, genotip GG'ye kıyasla genotip AA'da bir mutasyon (287 A->G) olduğunu gösterdi. BMPR1B geninin A287G SNP'si, vücut ağırlığı ile 2. (P=0.022), 3. (P=0.034), 4. (P=0.011), 5. (P=0.035), 6. (P=0.001), 7. (P=0.008) ve 8. haftalarda (P=0.016) önemli ölçüde ilişkili idi. Sonuç olarak, BMPR-1B geni tavuklarda vücut ağırlığı ile ilişkili olabilir ve Markör Destekli Seçim programında tavukların büyüme performansını artırmak için dikkate alınabilir

Tavuklarda Kemik Morfogenetik Protein Reseptörü 1B (BMPR-1B) Genindeki Tek Nükleotid Polimorfizmi ile Büyüme Özellikleri Arasındaki İlişki

Growth traits are under the control of multiple genes. Understanding the genetic information of related genes is helpful for the selection and breeding course through marker assisted selection. The aim of the current study was to investigate the association of A287G SNP in BMPR-1B gene with growth traits in chicken. A single nucleotide polymorphism was identified in 240 individuals using the PCR-RFLP technique and confirmed by sequencing. The allelic and genotypic frequencies were compared, using the Chisquared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model of statistical software SAS. Three genotypes (AA, AG and GG) were detected in Fayoumi and Rhold Island Red chicken. Sequencing revealed one mutation (287 A->G) in the genotype AA in comparison to the genotype GG. The A287G SNP of BMPR-1B gene was associated significantly with body weight at 2nd (P=0.022), 3rd (P=0.034), 4th (P=0.011), 5th (P=0.035), 6th (P=0.001), 7th (P=0.008) and 8th(P=0.016) week of age. In conclusion, BMPR-1B gene may be associated with body weight in chicken and may be considered in Marker Assisted Selection program to improve chicken growth performance

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