Erzurum yöresinde üst solunum yolu enfeksiyonlu hastalarda bazı viral etkenlerin araştırılması

Bu çalışmada, hücre kültürü yöntemi ile üst solunum yollarında infeksiyon oluşturan İnfluenzavirus, Adenovirus (ADV), Parainfluenzavirus, Respiratory Syncytial Virus (RSV) saptanması ve Erzurum yöresindeki prevalansının belirlenmesi amaçlandı. Araştırmada 97’si (%56.7) yetişkin, 74’ü (%43.3) çocuk olmak üzere toplam 171 hastaya ait nazofaringeal swap örneği kullanıldı. Yetişkin örnek grubunun 49’unu (%50.5) erkekler, 48’ini ise (%49.5) kadınlar oluştururken, çocuk örnek grubunun 33 adedini (%44.6) erkek çocuklar, 41 adedini de (%55.4) kız çocuklar oluşturmaktaydı. Çalışma sonucunda hiçbir numunede Parainfluenza virusuna rastlanmazken, 27 örnekte (10 çocuk, 17 erişkin; %15.8) İnfluenzavirus A, 11 örnekte (10 çocuk, 1 erişkin; %6.4) ADV, 7 örnekte (6 çocuk, 1 erişkin; %4.1) RSV ve 5 örnekte de (4 çocuk, 1 erişkin; %2.9) İnfluenzavirus B tespit edildi. Kuş gribi (H5N1) şüpheli bir çocuk hastada da Real Time PCR yöntemi ile Roboscreen RoboGene H5N1 hazır ticari kiti ile RNA araştırılması yapıldı ve hastada H5N1 nükleik asidi tespit edildi Ayrıca marazi maddelerin MDCK (İnfluenzavirus A-B) ve Hep-2 (RSV, ADV) hücrelerine ekimleri yapılarak Immun floresans antikor yöntemi ile viral etkenlerin varlığı araştırıldı. Viral etkenler arasında en fazla İnfluenzavirus A saptanırken, en az İnfluenzavirus B tespit edildi. Çalışmamızda hastaların yaş grupları ve cinsiyetler arasında viral ajan görülme oranları bakımından istatistiksel bir fark bulunamadı.

Anahtar Kelimeler:

Türkiye, teşhis, hastalık yaygınlığı, hastalık tetkiki, epidemiyoloji, insan hastalıkları, influenza A, influenza B, parainfluenza, polimeraz zincir reaksiyonu, solunum yolu hastalıkları, sığır solunum yolu sinsitiyal virüsü, insan

The investigation of some viral agents in the patients with upper respiratory tract in Erzurum region

In this study, we aimed to determine the prevalance of Respiratory Syncytial Virus, Parainfluenza and Adenovirus leading to infection and influenza in upper respiratory tract by means of cell culture method. In the study, samples belonging to total 171 patients, 97 of whom are adults (56.7%) and 74 of whom are children (43.3%) were used. While 49 of adult sampling group were male (50.5%), 48 of them were female (49.5%), and children sampling group consisted of 33 male (44.6%) and 41 female (55.4%). In the conclusion of the study, while parainfluenza virus couldnt be observed, there was Influenza A in 27 samples (10 children, 17 adults; 15.8%), and there was Adenovirus (ADV) in 11 samples (10 children and 1 adult; 6.4%), and there was Respiratory Syncytial Virus (RSV) in seen 7 samples (6 children and 1 adult; 4.1%) and there was Influenza B virus in 5 samples (4 children and 1 adult; 2.9%) and H5N1 Bird Flu Virus was detected in a patient by means of Real Time PCR method. By means of Roboscreen RoboGene H5N1 Ready-Commercial Kit, RNA investigation was carried out ın one sample (a children) H5N1 virus was detected. After planting for MDCK and Hep-2 cells, and by using direct immunoflourescence (IFA) method in order for cytopotic effects to be devided, virus agents were detected. By means of cell culture, Influenza A and B were produced in MDCK cells, and RSV and ADV were produced in Hep-2 cells. Among viral agents, while Influenza A was detected the most, Influenza B was found the least. In our study, we couldnt find a distinction in the rates of viral agents prevalence according to age and genders of the patients by means of chi-square method.

Keywords:

Turkey, diagnosis, disease prevalence, disease surveys, epidemiology, human diseases, influenza A, influenza B, parainfluenza, polymerase chain reaction, respiratory diseases, bovine respiratory syncytial virus, man,

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