Effects of Sperm from Different Bulls on Developmental Competence, Blastosist Quality and Cell Number of Bovine Embryos In Vitro

Bu çalışma farklı boğa spermalarının sığır embriyolarının in vitro gelişim yetkinliği, blastosist kalitesi ve hücre sayısı üzerine etkilerini belirlemek amacıyla yapılmıştır. İn vitro olgunlaştırılan sığır oositleri, 5 farklı Avusturyan Simental Fleckvieh boğasına ait dondurulmuşçözdürülmüş boğa sperması ile fertilize edilmiş ve sonra sentetik ovidukt sıvısı medyumu içerisinde yüksek oranda nemlendirilmiş 38,5°C, %5 CO2, %5 O2 ve %90 N2 içeren atmosferde 8 gün boyunca kültüre alınmıştır. Sunulan çalışmada, embriyoların bölünme, morula, morula/bölünme, blastosist ve blastosist/bölünme oranları üzerine boğa çeşitliliğinin etkisi tespit edilmemiştir. Ayrıca beş farklı boğa sperması ile in vitro fertilize edilmiş oositlerden elde edilen blastosistlerin İHK hücre sayısı ve İHK/toplam hücre oranı benzer bulunmuştur. Ancak boğa 5 sperması ile in vitro fertilize edilmiş oositlerden elde edilen blastosistlerin trofektoderm ve toplam hücre sayısı diğer boğalardan daha düşük olduğu belirlenmiştir (P

Farklı Boğa Spermalarının Sığır Embriyolarının İn Vitro Gelişim Yetkinliği, Blastosist Kalitesi ve Hücre Sayısı Üzerine Etkileri

The aim of this study was to investigate the effects of sperm from different bulls on the developmental competence, blastosist quality and cell number of bovine embryos in vitro. In vitro matured bovine oocytes were fertilized with frozen-thawed sperm from five different bulls of Austrian Simmental Fleckvieh and then cultured in Synthetic Oviduct Fluid (SOF) medium at 38.5°C, 5% CO2, 5% O2 and 90% N2 atmosphere with high humidity for 8 days. In the present study, there was no significant effect of bull variations on cleavage, morula, morula/cleavage, blastocyst and blastocyst/cleavage rates of embryos. Additionally, ICM cell numbers and ICM/total cells ratio of blastocysts obtained from in vitro fertilized oocytes with five bull's sperm were similar. However trophectoderm and total cell numbers of blastocysts obtained from in vitro fertilized oocytes with bull 5 sperm were lower than other bulls (P<0.05). In the present study, blastocysts quality was affected by bull variations and the percentage of excellent or good quality blastosist (Grade I) were lower (P<0.05) in embryos from fertilized oocytes with bull 5 sperm than those of other bulls, but the percentage of low quality blastosist (Grade III) were higher (P<0.05). There was a positive correlation between quality and total cell numbers of blastocysts in experimental groups. Result of present study showed that developmental competence of embryos in vitro were not affected by bulls variations, but may be influence blastocyst quality and cell numbers of blastocyst

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