Nebi ÇETİN, Funda EŞKİ, Leyla MİS, Zahid NASEER, Memiş BOLACALI

Dynamics of Oxidants, Antioxidants and Hormones During Diff erent Phases of Pregnancy in Hairy Goats

The aim of the present study was to observe the variation in oxidant (MDA), antioxidants (SOD, GSH, GSH-Px) and hormones (P4 and E2) levels in pregnant hairy goats during breeding season. In the this study, twenty hairy goats were synchronized by using sponges containing progesterone (fl uorogestone acetate). The animals showing oestrus were inseminated twice, first at 18th -24th h and second at 36th - 48th h of oestrus. On 35th and 42nd days after insemination, pregnancy diagnosis examination was performed with transrectal ultrasonography. The blood samples were collected from pregnant goats at 0, 11, 24 , 57, 100, 134, 141 days of gestation and immediately after the parturation. Serum samples collected at estrus and diff erent stages of gestation were analyzed for MDA, SOD, GSH-Px, GSH, P4 and E2 concentrations using standard protocols. The results showed that MDA level did not change in pregnant goats. The SOD, GSH and GSH-Px levels also shown a similar pattern throughout pregnancy period. The peak (P

Kıl Keçilerinde Gebeliğin Farklı Aşamalarında Oksidanların, Antioksidanların ve Hormonların Dinamikleri

Bu çalışmanın amacı, üreme sezonundaki gebe kıl keçilerinde oksidan (MDA), antioksidan (SOD, GSH, GSH-Px) ve hormon (P4 ve E2) seviyelerindeki farklılıkları gözlemlemekti. Bu çalışmada 20 adet kıl keçisi progesteron içeren (fl uorogeston asetat) vaginal süngerler kullanılarak senkronize edildi. Östrüs gösteren hayvanlar ilk olarak östrüsün 18-24. saatlerinde daha sonra 36-48. saatlerde olmak üzere iki defa tohumlandı. Tohumlama sonrası 35-42. günlerde transrektal ultrasonografi ile gebelik muayenesi yapıldı. Gebe keçilerden gebeliğin 0, 11, 24, 57, 100, 134, 141. günlerinde ve doğumdan hemen sonra kan örnekleri alındı. Gebeliğin farklı dönemlerinde toplanan serum örnekleri standart protokoller kullanılarak MDA, SOD, GSH-Px, GSH, P4 ve E2 konsantrasyonları için analiz edildi. Sonuçlar, MDA seviyesinin gebe keçilerde değişmediğini gösterdi. SOD, GSH ve GSH-Px seviyeleri de gebelik süresince benzer kaldı. Gebeliğin 11 ile 134. günleri arasında progesteron en yüksek seviyedeydi (P

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