Yingjie LU, Shuwen TAN, Bingqing O., Xiwen ZHANG, Ying YANG, Shujian HUANG, Saeed EL-ASHRAM

Inducible Nitric Oxide Synthase (iNOS) Enzyme Activity and Transcription Level as Well as Ultrastructural Changes in Different Tissues of Grass Carp in Response to Ichthyophthirius multifiliis

This study aims to contribute to this growing area of research by exploring the gene expression and enzyme activity of iNOS, and provide new insights into the ultrastructural changes in the kidneys, spleen, skin and gills infected with Ichthyophthirius on grass carp. Furthermore, electron microscopy (EM) findings illustrated the uneven distribution of the macrophages and lymphocytes, and the concentrated and fragmented and/or disappeared cell nuclei in the kidneys, spleen, gills and skin. The extreme ultrastructural changes resulted in the reduction in the number of immune cells and subsequent downregulation of iNOS enzyme activity, especially in the gills and skin of Ichthyophthirius-infected grass carp. Our results showed that enzyme activity and gene expression of iNOS were significantly down-regulated in the kidneys and spleen (P>0.05), gills and skin (P

Ichthyophthirius multifiliis’e Karşı Çim Sazanının Farklı Dokularında İndüklenebilir Nitrik Oksit Sentaz (iNOS) Enzim Aktivitesi, Transkripsiyon Seviyesi ve Ultrastrüktürel Değişiklikler

Bu çalışma Ichthyophthirius ile enfekte edilmiş Çim sazanının böbrek, dalak, deri ve kuluçkasında meydana gelen ultrastrüktürel değişiklikler ile iNOS enzim aktivitesi ve gen ekspresyonunu araştırmak suretiyle katkıda bulunmayı amaçlamaktadır. Böbrek, dalak, solungaç ve deride elektron mikroskopi bulguları makrofaj ve lenfositlerin dengesiz dağılımı ile konsantre ve parçalanmış ve/veya gözden kaybolmuş çekirdeğin olduğunu göstermiştir. Ichthyophthirius ile enfekte Çim sazanının özellikle solungaç ve derisinde aşırı ultrastrüktürel değişiklikler, immun hücrelerin sayısında azalma ve sonrasında iNOS enzim aktivitesinde aşağı yönde ayarlanma ile sonuçlandı. Elde edilen sonuçlar, iNOS enzim aktivitesi ve gen ekspresyonunun negatif kontrol grubu ile karşılaştırıldığında böbrek ve dalakta anlamlı derecede (P>0.05), solungaçlarda ve deride (P

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