Determination of the Tumor Virus B Locus in Turkish Native Chicken Breeds

Avian leukosis virusleri (ALV), tavuklarda tümör oluşturan retrovirüslerdir. Tipik olarak, ALV hayvan yetiştirme yöntemleri ile kontrol edilir. Bununla birlikte, genetik gelişme ve/veya selektif ıslah teknikleri, hastalığa direnç için benzersiz olanaklar sunmaktadır. Tümör viral B (TVB) lokusu ALV üç alt grubunun (B, D ve E) viral girişine ortam sağlayan/engelleyen veya aracı olan gruplara özgü yüzey reseptörlerini kodlar. Bu lokusun 172. ve 184. bazlarındaki iki adet tek nükleotit polimorfizmleri TVB*S1, TVB*S3 ve TVB*R allellerini ayırt edilmesine imkan sağlar. TVB*R'nin kodladığı reseptör ALVB, ALVD veya ALVE alt gruplarının hücreye viral girişini engeller. PCR-RFLP yöntemi kullanılarak Türkiye yerli tavuk ırkları olan Denizli ve Gerze tavuklarındaki TVB genotipleri belirlenmiştir. Hem Gerze hem de Denizli ırkında, TVB*S1 alleli yaygın ve TVB*S3 alleli nadir olarak görülmüştür. TVB*R alleli, Gerze popülasyonunda seyrek olarak görülürken Denizli horozu popülasyonlarında tespit edilememiştir. TVB*S1, TVB*S3 ve TVB*R'nin allel frekansları sırasıyla Gerze tavuklarında 0.96, 0.02 ve 0.02, Denizli horozlarında sırasıyla 0.98, 0.02 ve 0.00 olarak hesaplanmıştır

Türkiye Yerli Tavuk Irklarında Tümör Viral B Lokusunun Belirlenmesi

Avian leukosis viruses (ALV) are retroviruses that can induce tumors in chicken. Typically, ALV is controlled through husbandry. However, genetic improvement and/or, selective breeding techniques, offer the unique possibility of true resistance. The tumor virus B (TVB) locus transcribes the cellular receptor that mediates an infection through B, D, and E subgroups of avian leukosis virus. Two single nucleotide polymorphisms (SNPs) at nucleotide positions 172 and 184 of the TVB locus account for the three major alleles, TVB*S1, TVB*S3, and TVB*R. The receptor encoded by TVB*R allele prevents viral entry into the cell of ALVB, ALVD, or ALVE subgroups. In this study, both SNPs at the TVB locus of Turkish native chicken breeds were investigated using a PCR-RFLP technique to detect. In both Gerze and Denizli breeds, the TVB*S1 allele was common and TVB*S3 was rare, respectively. The TVB*R allele was rare in the Gerze population and absent in the Denizli population. Allele frequencies of TVB*S1, TVB*S3, and TVB*R were evaluated as 0.96, 0.02, and 0.02 in Gerze chickens and 0.98, 0.02, and 0.00, in Denizli chickens, respectively

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