Oğuz MERHAN, GENCAY TAŞKIN TAŞÇI, Kadir BOZUKLUHAN, NİLGÜN AYDIN

Determination of Oxidative Stress Index and Total Sialic Acid in Cattle Infested with Hypoderma spp.

Hypodermosis is a parasitic disease, which is caused by larvae of Hypoderma lineatum and Hypoderma bovis flies in cattle and causes damage on the skin. It causes economic losses due to decrease in meat and milk yield, growth deficiency and being of no use in leather processing as a result of perforation of the leather. The aim of this study was to define the oxidative-antioxidative capacity and total sialic acid levels in cattle with hypodermosis. A total of 90 cattle was used in the study, among those, 30 with hypodermosis and 60 antibody-negative healthy bovine sera were used according to ELISA test results to investigate the biochemical parameters. Biochemical analysis included total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), and total sialic acid (TSA). TAC value was found to be statistically significantly lower (P

Hypoderma spp. İle Enfeste Sığırlarda Oksidatif Stres İndeksi ve Total Siyalik Asit Düzeyinin Belirlenmesi

Hypodermosis, sığırlarda Hypoderma lineatum ve Hypoderma bovis tipi sineklerin larvaları tarafından oluşturulan ve deride hasara neden olan paraziter bir hastalıktır. Hayvanların et ve süt veriminde azalma, büyüme eksikliği ve derinin delinmesi sonucunda deri işlemede kullanılamaması gibi ekonomik kayıplara neden olur. Çalışmanın amacı Hypoderma ile enfeste sığırlarda oksidatif-antioksidatif kapasite ve total siyalik asit düzeyinin belirlenmesidir. Çalışmada, ELISA test sonuçlarına göre, 60 antikor negatif ve 30 hypodermosisli olmak üzere toplam 90 adet sığır serumu kullanıldı. Biyokimyasal olarak total oksidan kapasite (TOK), total antioksidan kapasite (TAK), oksidatif stres indeksi (OSİ) ve total siyalik asit (TSA) analizi yapıldı. Kontrol grubu ile hypodermosisli sığırlar karşılaştırıldığında istatistiksel olarak TOK, OSİ ve TSA düzeyinin arttığı (P

PDF

___

1. Özkutlu Z, Sevgili M: Seroprevalence of hypodermatosis in cattle in the province of Sanliurfa (Turkey). Acta Parasitol Turcica, 29, 275-279, 2005.

2. Constable PD, Hinchcliff KW, Done SH, Grünberg W: Diseases of the skin, eye, conjunctiva, and external ear. In, Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats. 11th ed., 1626-1628, Elsevier, China, 2017.

3. Boulard C: Durably controlling bovine hypodermosis. Vet Res, 33, 455- 464, 2002. DOI: 10.1051/vetres:2002032

4. Hassan M, Khan MN, Abubakar M, Waheed HM, Iqbal Z, Hussain M: Bovine hypodermosis - A global aspect. Trop Anim Health Prod, 42, 1615- 1625, 2010. DOI: 10.1007/s11250-010-9634-y

5. Schauer R, Kamerling JP: Exploration of the sialic acid world. Adv Carbohydr Chem Biochem, 75, 1-213, 2018. DOI: 10.1016/bs.accb. 2018.09.001

6. Traving C, Schauer R: Structure, function and metabolism of sialic acids. Cell Mol Life Sci, 54, 1330-1349, 1998. DOI: 10.1007/s000180050258

7. Merhan O, Özcan A: Investigation of serum ceruloplasmin and total sialic acid levels in geese. Kafkas Univ Vet Fak Derg, 10, 139-142, 2004.

8. Pearce OMT, Laubli H: Sialic acids in cancer biology and immunity. Glycobiology, 26, 111-128, 2016. DOI: 10.1093/glycob/cwv097

9. Yarım GF, Umur Ş, Açıcı M, Beyhan YE: Serum sialic acid levels in cattles with cystic echinococcosis. Ankara Univ Vet Fak Derg, 57, 61-63, 2010. DOI: 10.1501/Vetfak_0000002311

10. Karabulut H, Gülay MŞ: Serbest radikaller. MAKU Sag Bil Enst Derg, 4, 50-59, 2016.

11. Tabakoğlu E, Durgut R: Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri. AVKAE Derg, 3, 69-75, 2013.

12. Süleyman H, Gül V, Erhan E: Oksidatif stres ve doku hasarı. Erzincan Tıp Derg, 1, 1-4, 2018.

13. Bozukluhan K, Atakisi E, Atakisi O: Nitric oxide levels, total antioxidant and oxidant and capacity in cattle with foot-and-mouth-disease. Kafkas Univ Vet Fak Derg, 19, 179-181, 2013. DOI: 10.9775/kvfd.2012.7244

14. Celi P, Gabai G: Oxidant/antioxidant balance in animal nutrition and health: The role of protein oxidation. Front Vet Sci, 2, 1-13, 2015. DOI: 10.3389/fvets.2015.00048

15. Merhan O, Bozukluhan K, Kuru M, Büyük F, Özden Ö, Kükürt A: Investigation of oxidative stress index and lipid profile in cattle with Brucellosis. Kafkas Univ Vet Fak Derg, 23, 933-937, 2017. DOI: 10.9775/ kvfd.2017.18004

16. Aydoğdu U, Coşkun A, Başbuğ O, Ağaoğlu ZT: Evaluation of total oxidant-antioxidant status and oxidative stress index in dogs with parvoviral enteritis. F Ü Sağ Bil Vet Derg, 32, 161-164, 2018.

17. Karababa F, Yesilova Y, Turan E, Selek S, Altun H, Selek S: Impact of depressive symptoms on oxidative stress in patients with psoriasis. Redox Rep, 18, 51-55, 2013. DOI: 10.1179/1351000213Y.0000000039

18. Sydow G: A simplifield quick method for determination of sialic acid in serum. Biomed Biochim Acta, 44, 1721-1723, 1985.

19. SPSS: IBM SPSS Statistics for Windows, Version 20.0. Armonk, New York, 2011.

20. Chabaudie N, Villejoubert C, Boulard C: The response of cattle vaccinated with Hypodermin A to a natural infestation of Hypoderma bovis and Hypoderma lineatum. Int J Parasitol, 21, 859-862, 1991. DOI: 10.1016/0020-7519(91)90155-Z

21. Taşcı S, Değer S, Akgül Y: Hypodermosis in Van and around. Y Y Ü Vet Fak Derg, 5, 143-153, 1994.

22. Nicolas-Gaulard I, Moire N, İnra CB: Effect of the parasite enzyme, hypodermin A, on bovine lymphocyte proliferation and interleukin-2 production via the prostaglandin pathway. Immunology, 84, 160-165, 1995.

23. Colwell DD: Stage specific mortality and humoral immune responses during pulse and trickle infestations of the common cattle grub, Hypoderma lineatum (Diptera: Oestridae). Vet Parasitol, 99, 231-239, 2001. DOI: 10.1016/s0304-4017(01)00459-9

24. Lopez C, Colwell DD, Panadero R, Paz A, Perez J, Morrondo P, Diez P, Cascallana JL, Santamaria V, Bravo A: Skin immune responses in cattle after primary and secondary infections with Hypoderma lineatum (Diptera: Oestridae) larvae. Vet Immunol Immunopathol, 108, 285-294, 2005. DOI: 10.1016/j.vetimm.2005.06.001

25. Vazquez L, Dacal V, Lopez C, Diaz P, Morrondo P, Diez-Banos P, Panadero R: Antigen-specific antibody isotypes, lymphocyte subsets and cytokine profiles in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae). Vet Parasitol, 184, 230-237, 2012. DOI: 10.1016/j. vetpar.2011.09.013

26. Panadero R, Lopez C, Vazquez L, Diaz P, Perez A, Cabanelas E, Morrondo P, Diez-Banos P: Effect of reinfestations on systemic immune responses in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae). Vet Parasitol, 193, 238-244, 2013. DOI: 10.1016/j.vetpar.2012.11.017

27. Eguchi H, Ikeda Y, Ookawara T, Koyota S, Fujiwara N, Honke K, Wang PG, Taniguchi N, Suzuki K: Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x-mediated cell adhesion. Glycobiology, 15, 1094-1101, 2005. DOI: 10.1093/glycob/cwj003

28. Guzel M, Kontas Askar T, Kaya G, Atakişi E, Erbil Avcı G: Serum sialic acids, total antioxidant capacity, and adenosine deaminase activity in cattle with theileriosis and anaplasmosis. Bull Vet Inst Pulawy, 52, 227- 230, 2008.

29. Deveci HA, Kükürt A, Nur G, Alpay M, Merhan O, Bozukluhan K, Yilmaz V, Karapehlivan M: Serum paraoxonase activity and total sialic acid in sheep with foot and mouth disease. Med Weter, 74, 199-202, 2018. DOI: 10.21521/mw.6078

30. Keleş İ, Ertekin A, Karaca M, Ekin S, Akkan HA: Studies on serum sialic acid and lipid bound sialic acid in cattle with leptospirosis. YYÜ Vet Fak Derg, 11, 121-122, 2000.

31. Erel O: A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 37, 277-285, 2004. DOI: 10.1016/j.clinbiochem. 2003.11.015

32. Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38, 1103-1111, 2005. DOI: 10.1016/j. clinbiochem.2005.08.008

33. Bozukluhan K, Merhan O, Özcan A, Gökçe Hİ, Gökçe G: Investigation of the levels of serum haptoglobin, oxidative indicators and some biochemical parameters in calves naturally infected with Toxocara vitulorum. Ankara Univ Vet Fak Derg, 64, 75-79, 2017. DOI: 10.1501/ vetfak_0000002778

34. Bozukluhan K, Merhan O, Gökçe Hİ, Öğün M, Atakişi E, Kızıltepe Ş, Gökçe G: Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected sheeppox virus. Kafkas Univ Vet Fak Derg, 24, 437-441, 2018. DOI: 10.9775/ kvfd.2017.19167

35. Değer S, Değer Y, Ertekin A, Gül A, Biçek K, Özdal N: Determination of the status of lipid peroxidation and antioxidants in cattle infected with Dictyocaulus viviparus. Turkiye Parazitol Derg, 32, 234-237, 2008.

36. Ozkurt-Borazan G, Aktas MS, Camkerten I, Gokçen A, Ipek H, Sahin T, Uren Paksoy N: Erythrocyte superoxide dismutase, catalase activity and malondialdehit level in hypodermosis. J Anim Vet Adv, 10, 84- 86, 2011.

37. Merhan O, Bozukluhan K, Gokce HI: Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. J Hellenic Vet Med Soc, 68, 535-540, 2017. DOI: 10.12681/ jhvms.16049