Rickettsia aeschlimannii and Wolbachia endosymbiont in Ctenocephalides canis from Eurasian lynx (Lynx lynx) Near the China-Kazakhstan Border

Twenty-five dog fleas (Ctenocephalides canis) and five ticks identified as Hyalomma asiaticum were collected from a Eurasian lynx (Lynx lynx) pup in northwestern China. Molecular analyses of four genetic markers showed the presence of Rickettsia aeschlimannii DNA in 5 out of 20 fleas. Only rickettsial 17-kDa gene was detected in the blood sample of the lynx. In addition, 2 out of 20 fleas were positive to Wolbachia endosymbiont by targeting 16S rDNA while there was no Bartonella DNA found both in 5 ticks and 20 fleas by using gltA and 16S-23S ITS. Our findings suggest that i) C. canis parasitizing wild Eurasian lynx harbors R. aeschlimannii and Wolbachia endosymbiont in the China-Kazakhstan border, and ii) Wolbachia endosymbiont in present study is closer to that in C. canis infesting sheltered dogs in Turkey.

Çin-Kazakistan Sınır Bölgesinde Bir Avrasya Vaşağındaki (Lynx lynx) Ctenocephalides canis’te Saptanan Rickettsia aeschlimannii ve Wolbachia endosymbiont

Çin’in kuzeybatısında bir Avrasya vaşağı (Lynx lynx) yavrusundan yirmi beş adet köpek piresi (Ctenocephalides canis) ve Hyalomma asiaticum olarak tanımlanan beş adet kene toplandı. Dört genetik markerin moleküler analizi, 20 pirenin 5’inde Rickettsia aeschlimannii DNA’sının varlığını gösterdi. Vaşağın kan örneğinde ise sadece riketsiyal 17-kDa geni tespit edildi. Buna ek olarak, 20 pire’den 2’si Wolbachia endosymbiont’un hedef 16S rDNA’sı yönünden pozitifken, gltA ve 16S-23S ITS kullanılarak yapılan analizde 5 kenede ve 20 pirede Bartonella DNA’sına rastlanmadı. Bulgularımız, i) Çin-Kazakistan sınırında vahşi Avrasya vaşaklarını enfekte eden C. canis’in R. aeschlimannii ve Wolbachia endosymbiont’a konakçılık yaptığını ve ii) mevcut çalışmadaki Wolbachia endosymbiont’unun, Türkiye’deki barınak köpeklerini enfekte eden C. canis’teki ile daha yakın olduğunu göstermektedir.

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Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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