İSMAİL AYTEKİN, NURİ MAMAK, AYKUT ULUCAN, ASLAN KALINBACAK

Clinical, haematological, biochemical and pathological findings in lambs with Peste des Petits Ruminants

Bu çalışmada competitive enzyme linked immunosorbet assay (c-ELISA) testi kullanılarak Peste des petits ruminants (PPR) tespit edilen Sakız melezi kuzularda, hastalığın klinik, hematolojik, biyokimyasal ve patolojik bulgularının belirlenmesi amaçlandı. Çalışmada 4-6 aylık yaşlarda ve farklı cinsiyetlerde peste des petits ruminants (PPR) ile enfekte 12 kuzu kullanıldı. Bununla birlikte, klinik olarak sağlıklı olan 6 kuzu da kontrol grubunu oluşturdu. Enfekte kuzularda yüksek ateş, anorexia, salivasyon, pnömoni, ishal, dudak, dil ve yanakta nekroz, eroziv ve nekrotik stomatit, purulent gözyaşı ve burun akıntısı gözlendi. Hematolojik muayenede infekte grupta, lökosit (WBCs), ortalama korpusküler volüm (MCV), ortalama korpusküler hemoglobin (MCH) ve ortalama korpusküler hemoglobin konsantrasyonu (MCHC) parametreleri anlamlı olarak arttı, fakat hemoglobin (Hb), eritrosit (RBCs), trombosit (PLT) ve hematokrit (PCV) parametreleri azaldı. Biyokimyasal muayene sonuçlarına göre, enfekte grupta serum gamma glutamil transferaz (GGT), alanin aminotransferaz (ALT) ve aspartat transaminaz (AST) konsantrasyonları, kontrol grubuyla karşılaştırıldığında anlamlı olarak yüksekti. Histopatolojik incelemede, akciğerde bronşitis, bronşiolitis ve alveol lümenlerinde sinsitiyal hücre oluşumları ve ayrıca, sinsitiyal hücreleri de içeren eroziv stomatitis gözlendi. Bunun yanısıra, nüklear ya da sitoplazmik yerleşimli, hem epitel hem de sinsitial yerleşimli viral antijenler belirlendi. İmmunohistokimyasal incelemede, virus ilişkili antijenler sıklıkla sitoplazmada ve nadiren de çekirdekte bulundu. PPR ile enfekte kuzularda yapılan bu çalışmanın sonuçları; hastalığın klinik, hematolojik, biyokimyasal ve histopatolojik bulguları hakkında önemli bilgiler sağladı.

Anahtar Kelimeler:

kan kimyası, klinik görünüm, melez ırklar, alyuvar sayısı, gama-glutamiltransferaz, hematoloji, hemoglobin, histopatoloji, bulaşıcı hastalıklar, akyuvar sayısı, koyun, viral hastalıklar, kuzu, alanin aminotransferaz, hayvan patolojisi

Peste des petits ruminants’lı kuzularda klinik, hematolojik, biyokimyasal ve patolojik bulgular

The aim of this study was to determine clinical, haematological, biochemical and pathological findings in Sakız crossbred lambs with Peste des Petits Ruminants, using a competitive enzyme linked immunosorbet assay (c-ELISA). In the study, 12 lambs infected with PPR 4 to 6 months age and dif erent sexes were used. Furthermore, 6 clinically healthy lambs were also used as control group. High fever, anorexia, salivation, pneumonia, diarrhea, erosive or necrotic stomatitis on the mucosae of lips, cheek and tongue, purulent ocular and nasal discharges were observed in infected lambs. In the haematological examination, White blood cell (WBCs), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) parameters were significantly increased, but haemoglobin (Hb), red blood cell (RBCs), peak cell volume (PCV) and platelets (PLT) parameters were decreased in infected group. According to the biochemical examination, serum gamma glutamyl transferase (GGT), alanin aminotransferaz (ALT) and aspartate transaminase (AST) concentrations were significantly higher in infected group compared to those of control group. In the histopathological examinations, bronchitis, bronchiolitis, and syncytial cell formation in the lumen of alveoles, in lung and also erosive stomatitis with syncytial cells were seen. Furthermore, nuclear or cytoplasmic virus-associated inclusions were detected both epithelial and syncytial cells. In immunohistochemical examination, virus-associated antigens were found most frequently in the cytoplasm and rarely in the nucleus. Results of the present study indicated that infection with PPR in lambs provide valuable datas about clinical, haematological, biochemical and histopathological findings

Keywords:

blood chemistry, clinical aspects, crossbreds, erythrocyte count, gamma-glutamyltransferase, haematology, haemoglobin, histopathology, infectious diseases, leukocyte count, sheep, viral diseases, lambs, alanine aminotransferase, animal pathology,

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