NADİDE NABİL KAMİLOĞLU, ALKAN KAMİLOĞLU, Ebru BEYTUT

Changes in antioxidant sytem, lipid peroxidation, Heart and respiratory rate and rectal temperature with ketamine and ketamine-xylazine anaestesia in tuj rams

Bu çalışma, ketamin ve ketamin-xylazinin koyunlarda kullanılan anestezik dozunun plazma ve eritrosit antioksidan sistemi ve thiobarbituric acid substance (TBARS) düzeyleri üzerine etkilerini belirlemek amacı ile 3-4 yaşında, ortalama 56-60 kg ağırlığında 16 klinik olarak sağlSklS koç üzerinde yürütüldü. Her grupta 8 koç olacak şekilde iki grup oluşturuldu. Anestezi oluşturmak maksadıyla I. Gruba 10 mg/kg ketamin HCl, II. Gruba 10 mg/kg ketamin HCl ve 50 μg/kg Xylazin HCl IM yolla enjekte edildi. Plazma E vitamini ve TBARS olarak lipit peroksidasyon düzeylerini, glutatyon peroksidaz (GSHPX) aktivitesini ve eritrosit glutatyon (GSH) düzeylerini belirlemek için, kan numuneleri ketamin ve ketamin-xylazin enjeksiyonundan önce ve 5, 10, 15, 30 ve 60. dakikalarda v. jugularis’ten alındı. Kalp hızı, solunum sayısı ve rektal ısı da aynı zaman aralıklarında 60 dak süresince belirlendi. I. Grupta plazma TBARS ve E vitamini düzeylerinin ketamin enjeksiyonundan 15 dak sonra azaldığı (P

Anahtar Kelimeler:

ksilazin, anestetikler, ağrı kesiciler, antioksidanlar, kalp hızı, ketamin, lipit peroksidasyonu, solunum hızı, koyun

Koyunlarda ketamin ve ketamin-xylazin anestezisi ile eritrosit antioksidan sistemi, lipid peroksidasyon, kalp hızı, solunum sayısı ve rektal ısıda meydana gelen değişiklikler

The objective of this study was to determine if a ketamine or ketamine-xylazine had any effect on the antioxidant defence system and on lipid peroxidation as thiobarbituric acid substance (TBARS) in rams. The experiment was carried out on 16 clinically healthy Tuj rams each weighing an average 56-60 kg and aged 3-4 years old. Rams were divided into two groups of eight ram in each. To produce anesthesia the Group I was given 10 mg/kg ketamine HCl, the Group II received 50 μg/kg xylazine HCl and 10 mg/kg ketamine HCl, intramuscularly. Blood samples were taken from the jugular vein before and at 5, 10, 15, 30 and 60th minutes after drugs injection to measure the levels of vitamin E and TBARS in plasma and the activity of glutathione peroxidase (GSHPX) and the levels of glutathione (GSH) in erythrocytes. Heart and respiratory rates and rectal temperature were also recorded for 60 min. in two groups. Although plasma levels of TBARS and vitamin E were significantly (P<0.001) lowered at the 15th min. after ketamine injection, the activity of GSHPx and levels of GSH in RBC were significantly increased at the 10th min. after ketamine injection (P<0.001) in Group I. While significant changes were observed in heart rate and respiratory rate at the 5th minute after anestesia. Also, the activity of GSHPx and levels of GSH in RBC in Group II were significantly increased at the 15th min. after ketaminexylazine injection (P<0.001). Levels of TBARS in plasma and vitamin E in plasma were not changed with ketamine-xylazine injection. Heart and respiratory rates began to increase at the 10th min. after ketamine-xylazine injection and decreased to a value approximately that of before analgesia by the 15th min. and remained stable thoughout the anaesthesia. The plasma levels of TBARS in the ketamine group were lover than those in the ketamine-xylazine group at the 15 and 30th min. after injections (P<0.001). GSH levels and GSHPx activity in RBCs of rams have a similar increase in two groups. There were no significant differences observed in rectal temperature during anaesthesia in Group I and II. In conclusion, the results of this study shows that ketamine and kombination of ketamine-xylazine injection at a therapeutic concentration can suppress free radical generation and support antioxidant system of rams.

Keywords:

xylazine, anaesthetics, analgesics, antioxidants, heart rate, ketamine, lipid peroxidation, respiration rate, sheep,

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