Ebru BEYTUT, NADİDE NABİL KAMİLOĞLU

Brucella abortus ile enfekte sığırların eritrosit antioksidan savunma sistemi ve lipid peroksidasyonundaki değişiklikler

Bu çalışmada, Brusella abortus (B. abortus) ile enfekte olmuş sığırların eritrositlerindeki redükte glutatyon (GSH), lipid peroksidasyonunun bir indikatörü olan tiyobarbiturik asit substratları (TBARS) düzeyleri, glutatyon peroksidaz aktivitesi (GSH-Px, EC. 1.11.1.9), ile plazma TBARS , $\beta$ -karoten, A ve E vitaminlerinin düzeylerim belirlemek amaçlanmıştır. Bunun için, Kars ve yöresinin değişik köylerinden ve bölgedeki iki kesim haneden sağlanan 20 sağlıklı ve 20 adet ikinci trimestırda abort yapmış ve serolojik testlerle B. abortus ile enfekte olduğu belirlenen toplam 40 sığırdan alınan kan numuneleri kullanılmıştır. B. abortus ile enfekte sığırların plazma TBARS düzeylerinin kontrollere göre istatistiksel olarak yüksek olduğu (p

Anahtar Kelimeler:

retinol, lipit peroksidasyonu, antioksidanlar, savunma mekanizması, alyuvarlar, sığır, Brucella abortus, E vitamini, glutatyon, beta-karoten, glutatyon peroksidaz

Lipid peroxidadion and antiowidant defence system in erythrocyte of cattles infected with brucella abortus

The aim of this work was to determine the levels of $\beta$ -carotene, vitamin A, E and thiobarbituric acid reactive substances (TBARS) as indicator of lipid peroxidation in the plasma and the activity of glutathione peroxidase (GSH-Px, EC. 1.11.1.9) and the levels of reduced glutathione (GSH) and the TBARS in red blood cell of cattle infected with Brucella abortus (B. abortus). In this study, blood samples of 20 healthy and 20 heifers aborted in second trimaster of gestation found to be brucella-positive by serological tests were used. The cattle were brought from various villages in Kars region and slaughtered in two local abattoirs. Our results show that the TBARS levels in the cattle infected with B. abortus were significantly (p<0.01) increased and GSH-Px activity and GSH levels in the erythrocytes were significantly decreased as compared to the healthy cattle. Also, vitamin E, A and (3-carotene levels in the plasma of the infected animals were significantly decreased. These results indicate that B. abortus infection-induced oxidative stress was reduced the vitamin E, A and (3-carotene levels in plasma and GSH levels and GSH-Px activity in the erythrocytes of cattle.

Keywords:

retinol, lipid peroxidation, antioxidants, defence mechanisms, erythrocytes, cattle, Brucella abortus, vitamin E, glutathione, beta-carotene, glutathione peroxidase,

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