Farhad DEHKORDI SAFARPOOR, Faham KHAMESIPOUR, Manouchehr MOMENI

Brucella abortus and Brucella melitensis in Iranian bovine and bufalo semen samples: The first clinical trial on seasonal, senile and geographical distribution using culture, conventiona and real-time polymerase chain reaction assays

Sığır ve yaban sığırlarının semen örneklerinde Brusella türlerinin tespit ve tanımlanması amacıyla konvansiyonel yöntemler ve Gerçek Zamanlı Zincirleme Polimeraz Reaksiyonu kullanıldı. İranda değişik mevsimlerde 4 ana bölgeden toplam 91 adet sığır ve yaban sığırı semen örnekleri toplandı. Semen örneklerinin toplandığı hayvanlar 1 yaş, 1-2 yaş, 2-3 yaş ve 3 yaş üzeri olarak gruplandırıldı. Örneklerden kültür hazırlandı, konvansiyonel PCR amacıyla DNA ekstraksiyonu yapılarak Brusella türleri için IS711 kullanılarak pimerler dizayn edildi. Pozitif sonuç veren örnekler Brucella abortus ve Brucella melitensis yönünden incelendi. Sığırların %21.56sında yaban sığırların %14.28inde Brusella tespit edildi. Çalışılan bölgeler arasında Khozestan en yüksek Sistan Va Balochestan ise en düşük Brusella oranına sahipti. İlkbaharda toplanan örneklerde oran en yüksek olarak bulundu. En yüksek Brusella oranı 1 yaşın altındaki hayvanlardan toplanan semen örneklerinde tespit edildi. Brucella abortus 25 sığırda (%24.50) ve 14 yaban sığırında (%15,38) Brucella melitensis ise 4 sığırda (%3.92) ve 1 yaban sığırında (%1.09) belirlendi.

İranda sığır ve yaban sığırları semen örneklerinde Brucella abortus ve Brucella melitensis: Kültür, konvansiyonel ve real-time polimeraz zincir reaksiyonu kullanılarak mevsimsel, yaşa bağlı ve bölgesel dağılımı üzerine ilk klinik çalışma

Conventional and real-time PCR assays were developed for detection and identification of Brucella species in bovine and buffalo semen samples. Totally, 91 bovine and buffalo semen samples were collected from 4 major provinces of Iran in various seasons. The animals which their semen samples were collected for this study had less than 1 year old, 1-2 years old, 2-3 years old and more than 3 years old. Samples were cultured and DNA was extracted and novel primers have been designed using the IS711 target of Brucella species for conventional PCR. Positive results of PCR have been studied for presences of Brucella abortus and Brucella melitensis. Totally, 21.56% and 14.28% of bovine and buffalo semen samples, respectively, were positive for Brucella species. Khozestan had the highest while Sistan Va Balochestan had the lowest incidences of Brucella species in studied regions. Samples which were collected in spring season had the highest rate of infection. Also, samples which were collected from less than 1 year old bovine and buffalo semen samples had the highest incidence of Brucella species. Totally, 25 (24.50%) and 4 (3.92%) bovine semen samples and 14 (15.38%) and 1 (1.09%) buffalo semen samples were positive for Brucella abortus and Brucella melitensis.

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