İsmet ALKIŞ, Suat EKİN, Serkan YILDIRIM, Ahmet BAKIR, Gizem ESER, Mehmet FIRAT

Evaluation of the Protective Effect of Chlorogenic Acid and Rhabdosciadium anatolyi Against Cyclophosphamide-Induced Ovarian Toxicity in the Rat with Histopathological and Immunohistochemical Findings

Cyclophosphamide (CD) has a range of adverse effects on ovarian tissue in humans. It is widely an anticancer drug and used in autoimmune disorders. Also, CD produces reactive oxygen species. In the current study, we evaluated possible protective roles of Rhabdosciadium anatolyi (RA) and chlorogenic acid (CA) on histopathological alterations and immunohistochemical assessment of 8-OHdG in the ovarian tissues of female rats given cyclophosphamide. Female Wistar albino rats were divided into six experimental groups, each consisted of eight rats: control group, CD (200 mg/kg) with i.p. for the first day as single dose, CA (100 mg/kg), RA (300 mg/kg) for each day, RA (300 mg/kg) + CD (200 mg/kg) and CA(100 mg/kg) + CD (200 mg/kg) groups. The rats were administered treatments lasted 7 days for all groups. CA and RA treatment is associated with positive the ovary action CD-induced ovotoxicity in rats. CA and RA could ameliorate the histopathological and immunohistochemical finding restoring which may show moderate levels of primordial follicle, primary, secondary, tertiary and graafian follicles were observed degeneration in germinative cells. Therefore, our results suggest that chlorogenic acid and Rhabdosciadium anatolyi might be a protective effect for CD-induced ovotoxicity.

Klorojenik Asit ve Rhabdosciadium anatolyi’nin Siklofosfamid İle Ratlarda Oluşturulan Ovaryum Toksisitesine Karşı Koruyucu Etkisinin Histopatolojik ve İmmunohistokimyasal Bulgularla Değerlendirilmesi

Siklofosfamid (CD), insanlarda ovaryum dokusu üzerinde bir dizi yan etkilere sahiptir. Yaygın bir şekilde antikanser ilacı olarak ve otoimmün hastalıklarda kullanılır. Ayrıca, CD, reaktif oksijen türleri üretir. Bu çalışmada, siklofosfamid verilen dişi ratların over dokularında, Rhabdosciadium anatolyi (RA) ve klorojenik asidin (CA) histopatolojik değişiklikler ve immünohistokimyasal olarak belirlenen 8-OHdG üzerine olası koruyucu rolleri değerlendirildi. Dişi Wistar albino ratlar, her grupta sekiz olacak şekilde, altı deney grubuna ayrıldı: kontrol grubu, i.p olarak ilk gün için tek doz CD (200 mg/kg), CA (100 mg/kg) ve RA (300 mg/kg) için her gün, RA (300 mg/kg) + CD (200 mg/kg) ve CA (100 mg/kg) + CD (200 mg/kg) grupları. Ratlara tüm gruplar için işlemler 7 gün süreyle uygulandı. CA ve RA uygulaması, ratlarda CD’nin neden olduğu ovotoksisite, over üzerine olumlu etkisi olması ile ilişkilidir. Germitif hücrelerinde gözlenen dejenerasyonu, aynı zamanda orta düzeyde görülen primordial follikül, primer, sekonder, tersiyer ve graf folliküllerin histopatolojik ve immünohistokimyasal bulgularının düzelmesini CA ve RA sağlayabilir. Bu nedenle sonuçlarımız, CD’nin neden olduğu ovotoksisiteye karşı klorojenik asit ve rabdosciadium anatolyi’nin koruyucu bir etki oluşturabileceğini düşündürmektedir.

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