Türkiye Yerli Koyun Irklarında CAST Genine Ait Genetik Çeşitliliğin Belirlenmesi

Bu çalışmanın amacı, Türkiye yerli koyun ırklarında CAST genine ait genetik çeşitliliğin PCR-RFLP yöntemi ile belirlenmesidir. Çalışmada 6 farklı yerli koyun ırkı; Kıvırcık, İmroz, Karayaka, Hemsin, Morkaraman ve Karagül kullanılmıştır. Bu çalışma Karayaka, Morkaraman ve Hemsin koyun ırklarında CAST geni çeşitliliğini ortaya koyan ilk çalışmadır. DNA izolasyonu ve PZR ile yükseltgemenin ardından RFLP analizi MspI enzimi ile gerçekleştirilmiştir. %2'lik agaroz jel elektroforezi ile M (336bç ve 286bç) ve N (622bç) olmak üzere iki allel saptanmıştır. İstatistiksel hesaplamalar ile allel ve genotip frekansları ile beklenen (Ho) ve gözlenen (Ho) heterozigotluk değerleri ile Hardy Weinberg dengesine uyum durumları saptanmıştır. En yüksek M allel frekansı İmroz (%96), N allel frekansı ise Kıvırcık ırkında tespit edilmiştir. MN, MM ve NN genotip frekansları en yüksek olarak sırasıyla Kıvırcık (%60), İmroz (%92.6) ve Morkaraman (%7.1) ırklarında belirlenmiştir. Kıvırcık, İmroz, Karayaka ve Karagül ırklarında NN genotipi bulunamamıştır. Heterozigotluk değeri Kıvırcık ırkında en yüksek (%60) iken, İmroz ırkında en düşük (%7.4) olarak belirlenmiştir. Kıvırcık ve Hemsin dışında tüm ırklar Hardy-Weinberg dengesine uyumlu bulunmuştur. Bazı ırkların NN genotipini bulundurmaması ve Kıvırcık ırkının MN genotipi için en yüksek genotip frekansına sahip olması; yerli koyun ırklarının yetiştirildikleri bölgelerdeki seleksiyon sürecinden kaynaklanmış olabileceği düşünülebilir

Genetic Variability of CAST Gene in Native Sheep Breeds of Turkey [1]

The aim of this study is to determine the genetic variability of CAST gene in native sheep breeds of Turkey by PCR-RFLP method. Six different native sheep breeds; Kivircik, Imroz, Karayaka, Hemsin, Red Karaman and Karakul were used in this study. This study was the first report about CAST gene variation in Karayaka, Red Karaman and Hemsin sheep breeds. After DNA isolation and PCR amplification, RFLP was performed with MspI enzyme. Two alleles M (336bp and 286bp) and N (622bp) were identified on 2% agarose gel electrophoresis. Allel and genotype frequencies, observed (Ho) and expected heterozygosity (He) and deviation from Hardy Weinberg Equilibrium were estimated by statistical analyses. The frequency of M allele was highest in Imroz (96%) and N allele was identified most frequently in Kivircik (30%) breed. Highest frequencies of MN genotype were identified in Kivircik (60%), MM in Imroz (92.6%) and NN in Red Karaman (7.1%) breeds respecitvely. Kivircik, Imroz, Karayaka and Karakul breeds were null from NN genotype. Kivircik sheep showed the highest heterozygosity (60%) and Imroz had the lowest (7.4%). The highest heterozygosity value was identified in Kıvırcık (60%), the lowest in Imroz (7.4%). All breeds except Kivircik and Hemsin were found in Hardy-Weinberg equilibrium. Absence of NN genotype in some breeds and high frequency of MN genotype in Kivircik breed might be resulted from the selection process of native sheep breeds in their breeding regions

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