Antimicrobial susceptibility of bacteria isolated from uteri of thoroughbred mares with fertility problems

Kısraklarda endometritis yüksek ekonomik kayba sebep olan önemli kısırlık nedeni olup, çoğunlukla bakteriyel infeksiyonlar sonucunda ortaya çıkmaktadır. Bu çalışmanın amacı, Almanyada kısraklarda bakteriyel endometritis patojenlerini belirlemek ve klinik izolatların çeşitli antimikrobiyal ajanlara duyarlılıklarını araştırmaktır. Bu çalışmada Almanyada fertilite problemi olan kısraklardan toplanan 247 uterus numunesi mikrobiyal ve antibiyotik duyarlılığı yönünden değerlendirildi. Yüzellibir (%61.1) numune bakteriyolojik olarak pozitif bulundu. 331i bakteri ve biri mantar olmak üzere toplam 332 mikroorganizma izole edildi. Bakteri izolatların % 21.9u Escherichia coli (E. coli), %15.9u, α-hemolitik streptokok, %15.4ü ß-hemolitik streptokok, %12si Bacillus spp., %10.5i γ-hemolitik streptokok, %5.7i koliform bakteri ve %3ü Staphylococcus aureus olarak identifiye edildi. Geriye kalan suşlar (%15.6), 15 farklı bakteri türü ve bir mantar suşundan oluştu. Kısrakların fertilitesini en sık etkileyen bakteriler olarak ß-hemolitik streptokok ve E. coli etkenlerine karşı antibiyogram testi uygulandı. Bu bakterilerin, penisilin, tulatromisin, tetrasiklin, eritromisin, florfenikol, seftiofur, amoksisilin, amoksisilin/klavulanik asit, enrofloksasin, gentamisin, sefkuinom, kolistin, marbofloksasin, sulfametoksazol/trimetoprim olmak üzere toplam 14 antimikrobiyal maddeye karşı duyarlılığı test edildi. Bu çalışmada, ß-hemolitik streptokok suşlarının tamamı florfenikol, seftiofur, amoksisilin/klavulanik asit, amoksisilin, enrofloksasin, sefkuinom, marbofloksasin ve sulfametoksazol/trimetoprime duyarlı olduğu, %29.3ünün de kolistine karşı dirençli olduğu belirlendi. E. coli suşlarının tamamı penisilline, %50si de eritromisine dirençli bulunurken, tamamının florfenikol, seftiofur, amoksisilin, amoksisilin/klavulanik asit, enrofloksasin, gentamisin, sefkuinom, kolistin, marbofloksasin, sulfametoksazol/trimetoprime duyarlı olduğu saptandı.

Fertilite problemli damızlık kısrakların uteruslarından bakteri izolasyonu ve antimikrobiyal duyarlılık

Endometritis is an important cause of subfertility with high economic impact in mares and is mostly associated with bacterial infections. The aim of this study was to investigate bacterial pathogens in uteri and the susceptibility of some clinical isolates against several antimicrobial agents frequently used to control bacterial endometritis in mares in Germany. A total of 247 uterine swabs taken from mares with fertility problems were cultured to isolate bacteria and in vitro antimicrobial susceptibility of ß-hemolytic Streptococcus spp. and Escherichia coli (E. coli) strains was determined. Totally, 151 samples (61.1%) were found culture positive. A total of 332 microorganisms including 331 bacteria and one fungus were isolated from the samples. From the bacteria, 21,9%, 15.9%, 15.4%, 12%, 10.5%, 5.7% and 3.0% of the isolates were identified as Escherichia coli (E. coli), α- hemolytic streptococci, ß-hemolytic streptococci, Bacillus spp., γ-hemolytic streptococci, coliform bacteria and Staphylococcus aureus (Staph. aureus) respectively. The remaining isolates (15.6%) included 13 other bacterial species and one fungus. ß-hemolytic streptococci and E. coli strains were considered as frequently associated with fertility problems and antimicrobial susceptibility of these isolates against 14 antimicrobial agents including penicillin, tulathromycin, tetracycline, erythromycin, florfenicol, ceftiofur, amoxicillin, amoxicillin/clavulanic acid, enrofloxacin, gentamicin, cefquinome, colistin, marbofloxacin and sulfamethoxazole/trimethoprim was determined. All ß-hemolytic streptococci were found to be susceptible to penicillin, ceftiofur, amoxicillin/clavulanic acid, enrofloxacin, cefquinome, marbofloxacin and sulfamethoxazole/trimethoprim, whereas 29.3% of ß-hemolytic streptococci showed resistance against colistin. All E. coli strains and 50% of them were resistant to penicillin and erythromycin, respectively, wheres all E. coli isolates were sensitive to all other tested antimicrobial agents used in this study.

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