MAHMUT OK, İSMAİL ŞEN, HASAN GÜZELBEKTEŞ, MURAT BOYDAK, CENK ER, UĞUR AYDOĞDU, Ramazan YILDIZ

The Importance of concentrations of sorbitol dehydrogenase and glutamate dehydrogenase and B-mode ultrasonographic examination in the diagnosis of hepatic lipidosis in dairy cows

Bu çalışmanın amacı; sütçü sığırların yağlı karaciğer sendromunun teşhisinde B-mode ultrasonografinin önemini belirlemek, hastalığın tanısında ultrasonografik muayene ile karaciğer biyopsi örneklerinin histolojik bulguları ve bazı biyokimyasal parametre sonuçlarını karşılaştırmaktır. Bu çalışmada 15 orta hepatik lipidozisli, 15 şiddetli hepatik lipidozisli ve 6 sağlıklı Holştein ırkı sütçü sığır kullanıldı. Bütün sığırlardan kan alındı ve analiz edildi. Sığırların karaciğerinin ultrasonografik muayenesi yapıldı. Hepatik lipidozisli sığırlardan karaciğer biyopsisi alındı. Orta dereceli hepatik lipidozisli sığırlarda serum glutamate dehidrogenaz (GDH) ve aspartat aminotransferaz (AST) seviyeleri artarken, şiddetli hepatik lipidozisli sığırlarda serum sorbital dehidrogenaz (SDH), GDH ve AST seviyeleri arttı. Ultrasonografik maueyenede; orta ve şiddetli hepatik lipidozisli sığırlarda karaciğerde diffuz ekojenite artışı gözlemlenmekle birlikte, orta dereceli hepatik lipidozis olgularında ekojenite artışı daha azdı. Şiddetli hepatik lipidozisde SDH ve GDH enzim düzeylerinde, orta dereceli hepatik lipidozisde ise sadece GDH enzim düzeyinde artış belirlendi. Hem ultrasonografik muayene hem de karaciğerin spesifik enzim ölçümü hepatik lipidozisin teşhisinde faydalı olduğu düşüncesine varıldı.

Sütçü sığırların hepatik lipidozisinin teşhisinde B-mode ultrasonografik muayene ve serum sorbital dehidrogenaz ve glutamete dehidrogenaz düzeylerinin önemi

The aim of this study is to determine the importance of B-mode ultrasonography in the diagnosis of hepatic lipidosis in dairy cows and compare this mode of diagnosis with both the histologic examination of liver biopsy samples and investigation of some biochemical parameters associated with hepatic lipidosis. 15 Holstein cows with moderate hepatic lipidosis and 15 cows with severe hepatic lipidosis and 6 healtyh cows were used as a metarilas. Blood samples were obtained from all cows and analyzed. Liver samples were obtained by biopsy in cattle. The ultrasonographic examination of liver was performed on animal. Serum glutamate dehydrogenase (GDH) and aspartate amino transferase (AST) concentrations were increased in cows with moderate hepatic lipidosis. Serum sorbitol dehydrogenase (SDH), GDH, and AST concentrations were increased in cows with severe hepatic lipidosis. Ultrasonographic examination revealed an increase in diffuse echogenicity of the liver in cows with moderate and severe hepatic lipidosis cows, but the increase was little in moderate hepatic lipidosis. Both serum GDH and SDH levels were found to be increased in severe hepatic lipidosis. However, only the serum GDH level was elevated in moderate hepatic lipidosis. Therefore, both ultrasonographic examination and measurement of specific liver enzymes seem to be beneficial in the diagnosis of hepatic lipidosis.

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