İsmail TEMUR, KAHRAMAN ÜLKER, C. Şahin ERMUTLU, Abdülaziz GÜL, Ürfettin HÜSEYİNOĞLU, Mete CİHAN, Onur ATAKiŞİ, Mahmut SÖZMEN

Heterotopic allogenic and autogenic ovarian transplantation in rabbits: Assessment and comparison of the morphological and endocrine characteristics

Bu çalışmanın amacı, immün sistemi baskılanmış tavşanlara subkutan heterotopik allojenik ve otojenik yumurtalık naklinin endokrin ve morfolojik özelliklerinin değerlendirilmesi ve karşılaştırılmasıdır. Her gruba 7 tavşan dahil edildi. Grup I (allojenik: n=7) taze subkutan heterotopik allojenik transplantasyon (grup IIIden, grup Ie), grup II (otojenik: n=7) taze subkutan heterotopik otojenik transplantasyon (kendi ciltaltlarına) ve grup III donor grupları olarak belirlendi. Serum follikül stimülan hormone (FSH) düzeyleri, grup Ide, 2. haftada, 3. haftadaki değerlerden (P=0.017) anlamlı derecede daha düşüktü, ve grup IIde 4. haftadaki değerler, diğer haftalardaki değerlerden anlamlı olarak daha yüksekti (P=0.001). Serum 17-β estradiol (E2) düzeyleri, grup Ide, 3. haftada, 1. ve 2. haftalardaki değerlerden anlamlı derecede düşüktü (P=0.008) ve grup IIde 4. haftadaki değerler, diğer zamanlardaki değerlerden anlamlı derecede düşüktü (P=0.001). Serum progesterone (P4) düzeyleri, grup Ide, anlamlı farklılık göstermedi (P=0.441) ve grup IIde, 3. haftadaki değerlerden, 1. haftadaki değerler anlamlı olarak yüksekti, ANOVA testine göre (P=0.033). Histolojik inceleme Hematoksilen-eozin (HE) ile boyanarak ışık mikroskobu ile yapıldı. Bizim sonuçlarımız özellikle dört haftalık dönemde, FSH ve P4 düzeyleri açısından otojenik ve allojenik heterotopik taze nakledilen yumurtalık dokusu arasında anlamlı fark olmadığını göstermiştir. Sonuç olarak, otojenik ve allojenik taze nakledilen yumurtalık dokularının endokrin ve histolojik yönden benzer özelliklere sahip olduğu belirlenmiştir.

Tavşanlarda heterotopic allojenik ve otojenik yumurtalık nakli: Morfolojik ve endokrin özelliklerinin değerlendirilmesi ve karşılaştırılması

The aim of this study was to evaluate and compare the endocrine function and morphological characteristics of subcutaneous heterotopic allogenic and autogenic ovarian transplantation in immune-suppressed rabbits. Each group included seven rabbits. Group I (allogenic group) underwent freshly subcutaneous allogeneic heterotopic transplantation (n=7) (from group III to group I). Group II (autogenic group) underwent freshly subcutaneous autogenic heterotopic transplantation (n7).Group III was the donor group (n=7). The levels of serum follicle-stimulating hormone (FSH) were significantly lower during the 2nd week (P=0.017) than during the 3rd week in group I, and were significantly higher during the 4th week than at other times (P=0.001) in group II. The levels of serum 17-β estradiol (E2) were significantly lower during the 3rd week (P=0.008) than during the 1st and 2nd weeks in group I, and were significantly lower during the 4th week than at other times (P=0.001) in group II. The levels of serum progesterone (P4) were not vary significantly (P=0.441) in group I and were significantly higher during the 1st week than during the 3rd week (P=0.033) in group II according to the ANOVA. Histological examination was performed using light microscopy after staining with hematoxylin-eosin (HE). Our results showed that there is no remarkable difference between autogenic and allogeneic heterotopic freshly transplanted ovarian tissue, especially in terms of the FSH and P4 levels during a four week period. We found that autogenic and allogeneic freshly transplanted ovarian tissues had similar characteristics in terms of the endocrine and histological characteristics.

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