Abdulkadir KESKİN 1? Yasemin ÖNER 2 Gülnaz YILMAZBAŞ-MECİTOĞLU 1 Barış GÜNER 1 Ebru KARAKAYA 1 Cengiz ELMACI 2 Ahmet GÜMEN 1

Bu çalışmanın amacı fertil ve subfertil Siyah Alaca düvelerde aromataz sitokrom P450 (CYP19), östrojen reseptör ? (ER?), progesteron reseptör (PGR) genlerindeki bazı mutasyonların gen ve genotip frekanslarınınn PCR-RFLP yöntemi kullanılarak incelenmesi ve bu dağılımların fertil ve subfertil Siyah-Alaca düveler arasında karşılaştırılmasıdır. Çalışmaya toplam 106 düve dâhil edilmiş ve ilk tohumlamadan sonra gebe kalan düveler fertil düve grubu (n=51) olarak kullanılmıştır. Üç ve daha fazla tohumlama sayısına sahip düveler (n=55) subfertil olarak kabul edilmiştir. DNA izolasyonu için tüm düvelerden kan alınmıştır. PGR ve ER? genlerine ait lokuslarda iki allel ve üç genotip bulunurken, CYP19 geninde incelenen lokusta iki allel ve iki genotip belirlenmiştir. CYP19, ER?, PGR lokuslarında sırasıyla A alleli ve AA genotipi, G alleli ve GG, C alleli ve CT genotipi predominat olarak bulunmuştur. Ki- Kare (?2) test sonuçlarına göre incelenen her iki grupta tüm lokuslar bakımından Hardy- Weinberg dengesinde idi. Allel ve genotip frekansları bakımından fertil ve subfertil düveler arasında fark bulunamamıştır

Fertil ve Subfertil Siyah Alaca Düveler Arasında CYP19, ER?, PGR Allel Frekanslarının Dağılımı

The aim of this study was to investigate the gene and genotype distributions of some mutations in the aromatase cytochrome P450 (CYP19), estrogen receptor ? (ER?), and progesterone receptor (PGR) genes in fertile and subfertile Holstein-Friesian heifers using the PCR-RFLP method and comparing the distributions between groups. A total of 106 heifers were included the study, and the heifers that became pregnant after the first artificial insemination (n=51) were used as a fertile group. Heifers (n=55) with equal and more than 3 AIs were accepted as a subfertile heifers. Blood samples from all of the heifers were obtained for DNA isolation. While two alleles and three genotypes were found at the PGR and ER? loci, two alleles and two genotypes were detected at the CYP19 locus. The A allele and AA genotype, G allele and GG genotype, and C allele and CT genotype were found to be predominant in CYP19, ER? and PGR, respectively. According to the chi-square test (?2), two of the groups investigated were in Hardy-Weinberg equilibrium for all gene loci. There were no differences detected in allele or genotype frequencies between the fertile and subfertile heifers

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