Fatiha GACEM, Mohammed Amine MADADI, Nawel KHECHA, Rabah BAKOUR

Study of Vaccinal Properties of Clostridium chauvoei Strains Isolated During a Blackleg Outbreak in Cattle in Algeria

Bu çalışmanın amacı üç farklı Clostridium chauvoei Cezayir suşunun aşı özelliklerini araştırmaktır. Laboratuvar fermenterinde bir parça kültürü CAM medyumda yapıldı. Durağan-faz kültürün tümü formalinde inaktive edilere aşı üretildi. Suşların in vitro ve in vivo toksisiteleri, kültür spernatantlarının fare ve kobaylarda hemolitik aktivite ve letal etkileri ile değerlendirildi. Suşların patojeniteleri kültürün kobay ve farelere enjeksiyonu ile değerlendirildi. Pasif koruma deneyi anti-serum inoküle edilen ve virulent suş uygulanan fareler üzerinde yapıldı. Aşı kapasitesi, aşı inoküle edilen ve virulent suş uygulanan kobaylarda test edildi. ALG2 suşu daha büyük büyüme oranı (0.85 h-1) ve yapısal özellikleri ile diğer suşlardan ayırt edildi. ALG2 için patojenite ve toksisite gözlemlendi. Diğer suşlar için patojenite daha düşük ve in vivo toksisite yoktu. Pasif koruma testleri ALG2 den elde edilen immun serum için daha geniş koruyucu etki gösterdi. ALG2 suşundan hazırlanan aşı ile yapılan aktif koruma testi, 5 saha suşu ile muamele edilen aşılı kobayların hayatta kaldıklarını gösterdi. Bu çalışma Cezayir'de izole edilen C. chauvoei ALG2 suşunun immunojenik ve koruyucu kapasitelerini incelemiş ve aşı üretimi için uygun bir aday olabileceğini göstermiştir

Cezayir'de Sığırlarda Yanıkara Salgınında İzole Edilen Clostridium chauvoei Suşlarının Aşısal Özelliklerinin Araştırılması

The aim of this study was to investigate the vaccine properties of three Algerian strains of Clostridium chauvoei. Batch culture in laboratory fermenter was performed on CAM medium and Vaccine was obtained by formalin inactivation of whole stationary-phase culture. In vitro and in vivo toxicity of strains were evaluated, respectively, by the hemolytic activity and lethal effect on mice and guinea pigs of the culture supernatant. Pathogenicity of strains was assessed by injection of a culture to guinea pigs and mice. The passive protection assay was performed on mice inoculated with anti-serum and challenged with a virulent strain. Vaccine potency was tested on guinea pigs inoculated with vaccine and challenged with a virulent strain. The study of characteristics of growth distinguished the strain ALG2 from other strains by its greater growth rate (0.85 h-1) and structural integrity. Pathogenicity and toxicity were observed for ALG2, while pathogenicity was lower and in vivo toxicity was absent in other strains. Passive protection tests showed broader protective effect of immune sera from strain ALG2. Active protection testing with the vaccine prepared from strain ALG2 showed that all vaccinated guinea pigs challenged with five wild strains have survived. This study highlighted the immunogenicity and protective capacity of C. chauvoei strain ALG2 isolated in Algeria, which may be a good candidate for vaccine production

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