Fikriye POLAT, Nesrin TURAÇLAR, Eylem GÜL, Öztürk ÖZDEMİR, Günsel BİNGÖL

MUTATION ANALYSIS OF THE PROTO-ONCOGENES Ki-RAS AND C-MYC IN THE SOFT TISSUE TUMORS OF THE RATS THAT WERE FORMED BY 3-METHYLCHOLANTHRENE IN VIVO

Polisiklik aromatik hidrokarbonlar en potent karsinojenler olarak bilinmektedir. 3-Metilkolantren PAH’larıniçinde yer alan karsinojenik bir ajandır. 3-Metilkolantren’in memeli sistemlerinde deneysel olarak toksik vekarsinojenik etkileri gösterilmiştir. Bu bileşiklere çevrede zift, kurum, sıvı ve katı yakıtlar nedeniyle havada,ekzos gazında, sigara dumanında, dumanlanmış yiyeceklerde sıklıkla rastlanmaktadır. Bu çalışmanın amacı 3-Metilkolantren’ in 40 mg/kg’lık bir dozda kronik olarak ratlara verilmesiyle oluşan tümörlerde Ki-ras ekson 1, 2ve C-myc ekson 2 genlerinde herhangi bir mutasyona neden olup olmadığını tespit etmektir. Ratlar sonenjeksiyondan 26 hafta sonra servikal dislokasyon ile öldürüldü ve oluşan yumuşak doku tümörleri çıkarıldı.Dokular belirtilen genler için PCR tabanlı SSCP analiziyle incelendi. Sonuç olarak Ki-ras ekson 1, 2 ve C-mycekson 2 genlerinde hiçbir nokta mutasyonu görülmedi.

Anahtar Kelimeler:

Ki-ras geni, C-myc geni, 3-Metilkolantren, rat

MUTATION ANALYSIS OF THE PROTO-ONCOGENES Ki-RAS AND C-MYC IN THE SOFT TISSUE TUMORS OF THE RATS THAT WERE FORMED BY 3-METHYLCHOLANTHRENE IN VIVO

Keywords:

Ki-ras geni, C-myc geni, 3-Metilkolantren, rat,

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