In vitro preliminary studies of chitooligosaccharide coated nanostructured lipidic nanoparticles for efficient gene delivery
In vitro preliminary studies of chitooligosaccharide coated nanostructured lipidic nanoparticles for efficient gene delivery
Gene therapy is generally defined as the transfer of genetic material to cells to treat a disease or at least improve the clinical condition of a patient. The most commonly used vectors in gene therapy are viral and non-viral vectors. The aim of this study was to develop a gene carrier system based on chitooligosaccharide-coated nanostructured lipidic nanoparticles and evaluate the physicochemical properties, such as zeta potential, particle size,SEM images, pH, cytotoxicity, DNA-binding properties, serum stability and transfection to cells. In this study, cationic formulations were produced using Dynasan® 116 and Transcutol ®P as a lipidic phase and chitooligosaccharide lactate for polymeric coating with DOTAP as a cationic agent. These formulations were made with the oil-in water hot emulsification technique. GFP was selected as the genetic material to be loaded into the formulations. According to the results, the chitooligosaccharide-coated cationic lipid nanoparticles prepared had considerably small particle sizes (144-178 nm) and high zeta potential (+37.6/+33.7mV). Based on the MTT assay, the cytotoxic effect of formulations on the NIH 3T3, A549 and MDA-MB-231 cell lines exhibited a dose-time-dependant pattern. Further, the preparedformulations binded DNA effectively and protected DNA against the serum component. It was concluded that chitooligosaccharide-coated lipidic nanoparticle formulations can be prepared as a pDNA-nanoparticle complex and can be employed as a gene delivery system effectively.
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