Meltem ÇETİN, Afife, Büşra KANDİLLİ, Fatma DEMİRKAYA

Preparation and in vitro characterization of AL-Beads containing carbamazepine and/or levetiracetam

The purpose of the present study was to prepare only levetiracetam (LEV) or carbamazepine (CBZ) or their combination (LEV+CBZ)-loaded alginate (AL) beads for oral administration to treat epilepsy and to evaluate theirin vitro characteristics. AL beads were prepared using ionotropic gelation method and lyophilized. The bead formulations were evaluated for particles size, surface morphology, encapsulation efficiency and in vitro drug releasein PB pH 6.8 and HCl pH 1.2 release mediums. FT-IR was also used to characterize the beads. The mean particle sizes of wet and lyophilized AL beads were in range of 1.609±0.073-1.779±0.078 mm and 1.075±0.182-1.275±0.138 mm, respectively. The encapsulation efficiency (EE%) values of bead formulations were in the range of 18.74-24.65% for LEVand 90.36-94.03% for CBZ. The pH of the medium affects the swelling behaviours of AL beads and the degree of swelling of AL beads increased with increase of pH of release medium, due to exchange of the Ca2+ ion with Na+.Besides, the pH of the release medium and the high water solubility of LEV affected the drug release rate from beads. AL beads might be useful to use for oral administration of LEV and CBZ to obtain additive/synergistic effect in acombined treatment of epilepsy.

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