Mohammad Reza SHAKİBAİE, Gholamabas SALAJEGHEH, Yasser GOLKARİ

İran Kerman'da hastane kökenli Staphylococcus aureus suşlarında antimikrobiyalduyarlılık, virulans faktörleri ve biyofilm yapımı

Amaç: Bu çalışma hastane kaynaklı Staphylococcus aureus suşlarının antimicrobial duyarlılık, virülans faktör ve biyofilm oluşturma özelliklerini belirlemeyi hedeflemektedir. Metotlar: İran, Kerman'da 30 adet, tekrarlamayan S. aureus suşu hastanelerden izole edildi. Antimicrobial duyarlılıklar disk diffüzyon metoduyla CLSI rehberinin sınırdeğerlerine göre çalışıldı. Vankomisin ve metisilin için minimum inhibitor konsantrasyonu (MİK) sıvı mikrodilüsyon ve E-test prosedürleri ile belirlendi. Bu suşlarla birlikte olan virülans faktörleri (proteaz, DNaz, lesitinaz, kapsül ve hemoliz) çalışıldı. Biyofilm değerlendirmesi mikrotitre tekniği ile miktarı belirleme şeklinde yapıldı. Bulgular: Toplam olarak 14 (% 46, 7) S. aureus suşu alt solunum yollarından, altı suş (% 20,0) üriner sistemden ve kalan 10 suş (% 33,3) yaralar, kan ve ortopedi hastalarından izole edildi. Bütün suşlar tigesikline duyarlıydı, sekiz suş (% 26,7) metisiline dirençli (MRSA) bulundu ve dört suş (% 13,3) vankomisine azalmış duyarlılık gösterdi. Suşların hiç biri vankomisine dirençli değildi (p

Antimicrobial susceptibility, virulence factors and biofilm formation amongStaphylococcus aureus isolates from hospital infections in Kerman, Iran

Objective: The aims of present study were to determine the antimicrobial susceptibility, virulence factors and biofilm formation among MRSA hospital isolates of Staphylococcus aureus.Methods: Thirty non-repetitive strains of S. aureus isolated from three hospitals in Kerman, Iran. Antimicrobial susceptibility was determined by disk diffusion breakpoints method according to CLSI guideline. The minimum inhibitory concentration (MIC) of vancomycin and methicillin were measured by the broth microdilution and E-test procedures. Virulence factors (protease, DNase, lecithinase, capsule and hemolysis) associated with the above isolates was studied. Biofilm was quantified by microtiter technique.Results: In total, 14 (46.7%) S. aureus were isolated from lower respiratory tract, six (20.0%) from urinary tract and remaining 10 (33.3%) were recovered from wounds, blood and orthopedic patients. All of the isolates were susceptible to tigecycline, eight (26.7%) were found to be resistant to methicillin (MRSA) and 4 (13.3%) showed reduced susceptibility to vancomycin. No any vancomycin resistant isolate was detected (p

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