Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs
Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs
Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system. Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems. Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid. Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST.
___
- Metchock, B. G., F. S. Nolte, and R. J. Wallace III. Mycobacte- rium. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of Clinical Microbiology, p. 399-437, 7th ed. ASM Press, Washington, D.C, 1999.
- Baylan O, Kisa O, Albay A, Doganci L. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determined of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8: 772-777.
- Badak, F.Z., Kiosk, D.L., Setterquist, S., Hartley, C., O’Connell, M.A. and Hopfer, R.L., Comparison of Mycobacteria Growth Indicator Tube with BACTEC 460 for detection and recov- ery of mycobacteria from clinical specimens. J Clin Microbiol 1996; 34: 2236–2239.
- Rohner, P., Ninet, B., Metral, C., Emler, S. and Auckenthaler, R. Evaluation of the MB/BacT system in comparison to the BACTEC 460 system and solid media for isolation of myco- bacteria from clinical specimens. J Clin Microbiol 1997; 35: 3127–3131.
- Centers for Disease Control and Prevention (CDC) Essential components of a tuberculosis prevention and control pro- gram. Morbid Mortal Weekly Rep 1995; 44: 11-16.
- Rüsch-Gerdes S, Ebraihmzadeh A, Elliott LB, Hanna BA, Mor- gan MA, Novak S. Multicenter evaluation of the BACTEC MGIT 960 system compared to the BACTEC 460 TB and solid media for recovery of mycobacteria. In: Abstracts of the 19th Congress of the European Society of Mycobacteriology. Rev Port Pneumol 1998; 4: 346.
- Scarparo C, Ricordi P, Ruggiero G, Piccoli P. Evaluation of the fully automated BACTEC MGIT 960 system for testing sus- ceptibility of Mycobacterium tuberculosis to pyrazinamide, streptomycin, isoniazid, rifampin, and ethambutol and com- parison with the radiometric BACTEC 460TB method.J Clin Microbiol. 2004; 42:1109-1114.
- Kocagoz T, Alp A, Albay A. A new rapid non-radioactive me- dium for culturing mycobacteria, that also enables visual dif- ferentiation of mycobacterial growth from contamination (Ab- stract) . Los Angeles, CA: American Society of Microbiology General Meeting. May, 21-25, 2000.
- Ercis S, Alp A, Hascelik G, Kocagoz T. Comparison of Dio- TK rapid mycobacterium culture system with BACTEC 460 TB system and LJ medium in diagnosis of tuberculosis and detection of susceptibility of antituberculosis drugs (Abstract) Abant, Bolu, Turkey; 4th National Mycobacterium Sympo- sium, 2002: 180-181.
- Bicmen C, Coskun M, Senol G, Florat N, Kocagoz T. Com- parison of Dio-TK and LJ media for primary culture of my- cobacteria: a preliminary study for a new medium (Abstract) Glasgow, UK: 13th ECCMID, May, 2003.
- Baylan O, Kisa O, Albay A, L. Doganci. Evaluation of a new automated, rapid, colorimetric culture system using solid me- dium for laboratory diagnosis of tuberculosis and determina- tion of anti-tuberculosis drug susceptibility. Int J Tuberc Lung Dis 2004; 8:772–777.
- Solis LA, Shin SS, Han LL, Llanos F, Stowell M, Sloutsky A. Validation of a rapid method for detection of M.tuberculosis resistance to isoniazid and rifampin in Lima, Peru.Int J Tu- berc Lung Dis 2005; 9: 760-764.
- Negi SS, Khan SF, Gupta S, Pasha ST, Khare S, Lal S. Com- parison of the conventional diag-nostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis. Indian J Med Microbiol 2005;23:29-33.
- Ani A, Okpe S, Akambi M, Ejelionu E, Yakubu B, Owolodun O, Ekeh P, Oche A, Tyen D, Idoko J. Comparison of a DNA based PCR method with conventional methods for the detec- tion of M.tuberculosis in Jos, Nigeria. J Infect Dev Countries 2009; 3: 470-475.